Optimized Expression, Purification, and Rapid Detection of Recombinant Influenza Nucleoproteins Expressed in Sf9 Insect Cells.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of microbiology and biotechnology"
DOI: 10.0414/jmb.1805.05053
Abstract: Accurate and rapid diagnosis of influenza infection is essential to enable early antiviral treatment and reduce the mortality associated with seasonal and epidemic infections.Immunochromatography is one of the most common methods used for the diagnosis… read more here.
Keywords: detection; diagnosis; influenza; insect cells ... See more keywords
Effects of autophagy inducers on recombinant antibody production in insect cells
Sign Up to like & getrecommendations! Published in 2020 at "Cytotechnology"
DOI: 10.1007/s10616-020-00423-6
Abstract: Insect cells have recently proven to be an excellent platform for the high-level production of functional recombinant proteins. Autophagy is an important mechanism that promotes cell survival by eliminating damaged organelles and protein aggregates, and… read more here.
Keywords: production; antibody; insect cells; effects autophagy ... See more keywords
Production of influenza virus-like particles using recombinant insect cells
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2020.107757
Abstract: Abstract Virus-like particles (VLPs) are hollow nanoparticles composed of recombinant viral surface proteins without a virus genome. In the present study, we investigated the production of influenza VLPs using recombinant insect cells. DNA fragments encoding… read more here.
Keywords: insect cells; production influenza; influenza virus; virus ... See more keywords
LALLT (Loxosceles Allergen-Like Toxin) from the venom of Loxosceles intermedia: Recombinant expression in insect cells and characterization as a molecule with allergenic properties.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.08.212
Abstract: Bites evoked by Brown spiders (Loxosceles genus) are associated with skin injuries (cutaneous rash, itching, swelling, erythema and dermonecrosis) and systemic manifestations. Transcriptome analyses of Loxosceles venom glands showed that the venom has a complex… read more here.
Keywords: venom loxosceles; insect cells; toxin; loxosceles venoms ... See more keywords
Efficient production of antibody Fab fragment by transient gene expression in insect cells.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of bioscience and bioengineering"
DOI: 10.1016/j.jbiosc.2017.03.007
Abstract: Transient gene expression allows a rapid production of diverse recombinant proteins in early-stage preclinical and clinical developments of biologics. Insect cells have proven to be an excellent platform for the production of functional recombinant proteins.… read more here.
Keywords: production; transient gene; insect cells; gene expression ... See more keywords
Accurate Identification and Quantification of DNA Species by Next-Generation Sequencing in Adeno-Associated Viral Vectors Produced in Insect Cells.
Sign Up to like & getrecommendations! Published in 2017 at "Human gene therapy methods"
DOI: 10.1089/hgtb.2016.185
Abstract: Recombinant adeno-associated viral (rAAV) vectors have proven excellent tools for the treatment of many genetic diseases and other complex diseases. However, the illegitimate encapsidation of DNA contaminants within viral particles constitutes a major safety concern… read more here.
Keywords: quantification dna; dna; insect cells; raav ... See more keywords
Recombinant norovirus capsid protein VP1 (GII.4) expressed in H5 insect cells exhibits post-translational modifications with potential impact on lectin activity and vaccine design.
Sign Up to like & getrecommendations! Published in 2022 at "Glycobiology"
DOI: 10.1093/glycob/cwac017
Abstract: While surface proteins of most enveloped viruses are glycosylated, among non-enveloped viruses only few express glycoproteins in their capsid as infective virions. Noroviruses belong to the latter group and are known to express one major… read more here.
Keywords: capsid protein; post translational; protein vp1; insect cells ... See more keywords
Amino Alcohols from Eugenol as Potential Semisynthetic Insecticides: Chemical, Biological, and Computational Insights
Sign Up to like & getrecommendations! Published in 2021 at "Molecules"
DOI: 10.3390/molecules26216616
Abstract: A series of β-amino alcohols were prepared by the reaction of eugenol epoxide with aliphatic and aromatic amine nucleophiles. The synthesized compounds were fully characterized and evaluated as potential insecticides through the assessment of their… read more here.
Keywords: eugenol potential; semisynthetic insecticides; alcohols eugenol; insect cells ... See more keywords
Mammals Preferred: Reassortment of Batai and Bunyamwera orthobunyavirus Occurs in Mammalian but Not Insect Cells
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DOI: 10.3390/v13091702
Abstract: Reassortment is a viral genome-segment recomposition known for many viruses, including the orthobunyaviruses. The co-infection of a host cell with two viruses of the same serogroup, such as the Bunyamwera orthobunyavirus and the Batai orthobunyavirus,… read more here.
Keywords: bunyamwera orthobunyavirus; batai; insect cells; orthobunyavirus ... See more keywords
Gene Expression Analysis of Adapted Insect Cells during Influenza VLP Production Using RNA-Sequencing
Sign Up to like & getrecommendations! Published in 2022 at "Viruses"
DOI: 10.3390/v14102238
Abstract: Adaptive laboratory evolution has been used to improve production of influenza hemagglutinin (HA)-displaying virus-like particles (VLPs) in insect cells. However, little is known about the underlying biological mechanisms promoting higher HA-VLP expression in such adapted… read more here.
Keywords: cell; insect cells; expression; production ... See more keywords