Targeted, efficient sequence insertion and replacement in rice
Sign Up to like & getrecommendations! Published in 2020 at "Nature Biotechnology"
DOI: 10.1038/s41587-020-0581-5
Abstract: CRISPR–Cas9 methods have been applied to generate random insertions and deletions, large deletions, targeted insertions or replacements of short sequences, and precise base changes in plants 1 – 7 . However, versatile methods for targeted… read more here.
Keywords: replacement; insertion replacement; rice; modified donor ... See more keywords
Targeted Gene Insertion and Replacement in the Basidiomycete Ganoderma lucidum by Inactivation of Nonhomologous End Joining Using CRISPR/Cas9
Sign Up to like & getrecommendations! Published in 2021 at "Applied and Environmental Microbiology"
DOI: 10.1128/aem.01510-21
Abstract: Functional genomic studies in Ganoderma have been hindered by the absence of adequate genome-engineering tools. Although CRISPR/Cas9 works well for gene disruption and deletion in G. lucidum, targeted gene insertion and replacement have remained a… read more here.
Keywords: targeted gene; gene; insertion replacement; gene insertion ... See more keywords