High-throughput insertional mutagenesis reveals novel targets for enhancing lipid accumulation in Nannochloropsis oceanica.
Sign Up to like & getrecommendations! Published in 2021 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2021.04.012
Abstract: The microalga Nannochloropsis oceanica is considered a promising platform for the sustainable production of high-value lipids and biofuel feedstocks. However, current lipid yields of N. oceanica are too low for economic feasibility. Gaining fundamental insights… read more here.
Keywords: insertional mutagenesis; high throughput; nannochloropsis oceanica; nannochloropsis ... See more keywords
Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphisms.
Sign Up to like & getrecommendations! Published in 2022 at "Nature communications"
DOI: 10.1038/s41467-022-29228-1
Abstract: Due to epistasis, the same mutation can have drastically different phenotypic consequences in different individuals. This phenomenon is pertinent to precision medicine as well as antimicrobial drug development, but its general characteristics are largely unknown.… read more here.
Keywords: insertional mutagenesis; essentiality; transposon insertional; gene essentiality ... See more keywords
Transposon insertional mutagenesis in Saccharomyces uvarum reveals trans-acting effects influencing species-dependent essential genes.
Sign Up to like & getrecommendations! Published in 2019 at "Genome research"
DOI: 10.1101/gr.232330.117
Abstract: To understand how complex genetic networks perform and regulate diverse cellular processes, the function of each individual component must be defined. Comprehensive phenotypic studies of mutant alleles have been successful in model organisms in determining… read more here.
Keywords: species dependent; insertional mutagenesis; saccharomyces uvarum; essential genes ... See more keywords
TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii
Sign Up to like & getrecommendations! Published in 2020 at "PLoS ONE"
DOI: 10.1371/journal.pone.0232594
Abstract: Generation and subsequent analysis of mutants is critical to understanding the functions of genes and proteins. Here we describe TIM, an efficient, cost-effective, CRISPR-based targeted insertional mutagenesis method for the model organism Chlamydomonas reinhardtii. TIM… read more here.
Keywords: mutagenesis method; insertional mutagenesis; method; tim ... See more keywords
Insertional Mutagenesis Approaches and Their Use in Rice for Functional Genomics
Sign Up to like & getrecommendations! Published in 2019 at "Plants"
DOI: 10.3390/plants8090310
Abstract: Insertional mutagenesis is an indispensable tool for engendering a mutant population using exogenous DNA as the mutagen. The advancement in the next-generation sequencing platform has allowed for faster screening and analysis of generated mutated populations.… read more here.
Keywords: mutagenesis; mutagenesis approaches; functional genomics; insertional mutagenesis ... See more keywords