Articles with "insulated isothermal" as a keyword



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Type A Influenza Virus Detection from Horses by Real-Time RT-qPCR and Insulated Isothermal RT-PCR.

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Published in 2020 at "Methods in molecular biology"

DOI: 10.1007/978-1-0716-0346-8_29

Abstract: Equine influenza (EI) is a highly contagious disease of horses caused by the equine influenza virus (EIV) H3N8 subtype. EI is the most important respiratory virus infection of horses and can disrupt major equestrian events… read more here.

Keywords: detection; real time; insulated isothermal; influenza ... See more keywords

Development and validation of an insulated isothermal PCR assay for the rapid detection of Mannheimia haemolytica

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Published in 2022 at "Journal of Veterinary Diagnostic Investigation"

DOI: 10.1177/10406387211068447

Abstract: We developed a rapid insulated isothermal PCR (iiPCR) assay for on-site detection of Mannheimia haemolytica using a primer and probe set targeting the superoxide dismutase (sodA) gene. Our iiPCR assay detected M. haemolytica clinical isolates… read more here.

Keywords: haemolytica; detection; iipcr assay; isothermal pcr ... See more keywords

Development and validation of an insulated isothermal PCR assay for the rapid detection of Pasteurella multocida

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Published in 2024 at "Veterinarski arhiv"

DOI: 10.24099/vet.arhiv.2429

Abstract: Pasteurella multocida, a ubiquitous opportunistic pathogen, is renowned for causing diverse respiratory tract diseases in animals. In the quest for on-site detection solutions, we developed a rapid insulated isothermal PCR (iiPCR) assay, targeting the sodA… read more here.

Keywords: detection; insulated isothermal; pasteurella; development validation ... See more keywords

Rapid Detection of Fusarium oxysporum Using Insulated Isothermal PCR and a Rapid, Simple DNA Preparation Protocol

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Published in 2022 at "International Journal of Molecular Sciences"

DOI: 10.3390/ijms232113253

Abstract: We developed an insulated isothermal PCR (iiPCR) method for the efficient and rapid detection of Fusarium oxysporum (Fo), which is a fungus that infects various hosts and causes severe crop losses. The Fo iiPCR method… read more here.

Keywords: dna; detection; rapid detection; insulated isothermal ... See more keywords