Direct measurement of deubiquitinating enzyme activity in intact cells using a protease-resistant, cell-permeable, peptide-based reporter.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical engineering journal"
DOI: 10.1016/j.bej.2019.107320
Abstract: Deubiquitinating enzymes (DUBs) regulate the removal of the polyubiquitin chain from proteins targeted for degradation. Current approaches to quantify DUB activity are limited to test tube-based assays that incorporate enzymes or cell lysates, but not… read more here.
Keywords: cell permeable; activity; dub activity; peptide based ... See more keywords
Intact cells: "Nutritional capsules" in plant foods.
Sign Up to like & getrecommendations! Published in 2022 at "Comprehensive reviews in food science and food safety"
DOI: 10.1111/1541-4337.12904
Abstract: Macronutrients of pulses or cereals are stored in the cotyledon or endosperm cells with protection from intact cell walls. However, pulses and cereals are generally processed into fine particles during food production. For example, after… read more here.
Keywords: food; nutritional capsules; cells nutritional; cell ... See more keywords
Antioxidative effect of lactic acid bacteria: Intact cells vs. intracellular extracts
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Food and Drug Analysis"
DOI: 10.38212/2224-6614.2609
Abstract: The present study compared the anti-oxidative ability of intact cells and intracellular extracts of two lactic acid bacterial strains, Bifidobacterium longum and Lactobacillus delbrueckii ssp. bulgaricus. Results showed that both intact cells and intracellular extracts… read more here.
Keywords: ldl; effect; intact cells; cells intracellular ... See more keywords