Data-Independent Acquisition-Based Mass Spectrometry (DIA-MS) for Quantitative Analysis of Intact N-Linked Glycopeptides.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.1c01659
Abstract: N-linked protein glycosylation is a key regulator in various biological functions. Previous studies have shown that aberrant glycosylation is associated with many diseases. Therefore, it is essential to elucidate protein modifications of glycosylation by quantitatively… read more here.
Keywords: analysis; analysis intact; intact glycopeptides; linked glycopeptides ... See more keywords
Application of Microscopic Highly Hydrophilic Silica-Based Nanocomposites with High Surface Exposure in the Efficient Identification of Intact N-Glycopeptides.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00927
Abstract: Glycosylation of proteins regulates the life activities of organisms, while abnormalities of glycosylation sites and glycan structures occur in various serious diseases such as cancer. A separation and enrichment procedure is necessary to realize the… read more here.
Keywords: highly hydrophilic; microscopic highly; intact glycopeptides; surface ... See more keywords
Profiling of Endogenous Intact N-linked and O-linked Glycopeptides from Human Serum by Using an Integrated Platform.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00592
Abstract: Endogenous glycopeptides in serum are an invaluable resource for biomarker discovery. Due to the low abundance and the poor fragmentation in tandem mass spectrometry, the identification of endogenous intact glycopeptides still faces many challenges. Herein,… read more here.
Keywords: human serum; endogenous intact; integrated platform; intact glycopeptides ... See more keywords
A new energy setup strategy for intact N-glycopeptides characterization using higher-energy collisional dissociation.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.9b00089
Abstract: With the optional setting of multiple stepped collisional energies (NCEs), higher-energy collisional dissociation (HCD) as available on Orbitrap instruments is a widely adopted dissociation method for intact N-glycopeptide characterization, where peptide backbones and N-glycan moieties… read more here.
Keywords: energy; dissociation; collisional dissociation; energy collisional ... See more keywords
Capturing site-specific heterogeneity with large-scale N-glycoproteome analysis
Sign Up to like & getrecommendations! Published in 2019 at "Nature Communications"
DOI: 10.1038/s41467-019-09222-w
Abstract: Protein glycosylation is a highly important, yet poorly understood protein post-translational modification. Thousands of possible glycan structures and compositions create potential for tremendous site heterogeneity. A lack of suitable analytical methods for large-scale analyses of… read more here.
Keywords: large scale; site specific; heterogeneity; analysis ... See more keywords
Quantification of Intact O-Glycopeptides on Haptoglobin in Sera of Patients With Hepatocellular Carcinoma and Liver Cirrhosis
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2021.705341
Abstract: Haptoglobin (Hp) is one of the acute-phase response proteins secreted by the liver, and its aberrant N-glycosylation was previously reported in hepatocellular carcinoma (HCC). Limited studies on Hp O-glycosylation have been previously reported. In this… read more here.
Keywords: hepatocellular carcinoma; hcc; liver cirrhosis; glycosylation ... See more keywords
Glycoproteomic Analysis of Urinary Extracellular Vesicles for Biomarkers of Hepatocellular Carcinoma
Sign Up to like & getrecommendations! Published in 2023 at "Molecules"
DOI: 10.3390/molecules28031293
Abstract: Hepatocellular carcinoma (HCC) accounts for the most common form of primary liver cancer cases and constitutes a major health problem worldwide. The diagnosis of HCC is still challenging due to the low sensitivity and specificity… read more here.
Keywords: hepatocellular carcinoma; glycoproteomic analysis; extracellular vesicles; intact glycopeptides ... See more keywords