The benefits (and misfortunes) of SDS in top-down proteomics.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2017.03.002
Abstract: Top-down proteomics (TDP) has great potential for high throughput proteoform characterization. With significant advances in mass spectrometry (MS) instrumentation permitting tandem MS of large intact proteins, a limitation to the widespread adoption of TDP still… read more here.
Keywords: misfortunes sds; intact proteins; proteomics benefits; top proteomics ... See more keywords
Bridged Hybrid Monolithic Column Coupled to High-Resolution Mass Spectrometry for Top-Down Proteomics.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b05817
Abstract: Top-down mass spectrometry (MS)-based proteomics has become a powerful tool for comprehensive characterization of intact proteins. However, because of the high complexity of the proteome, highly effective separation of intact proteins from complex mixtures prior… read more here.
Keywords: column; mass spectrometry; intact proteins; separation ... See more keywords
MS-TAFI: A Tool for the Analysis of Fragment Ions Generated from Intact Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00594
Abstract: Tandem mass spectrometry (MS/MS) spectra of intact proteins can be difficult to interpret owing to the variety of fragment ion types and abundances. This information is crucial for maximizing the information derived from top-down mass… read more here.
Keywords: tool analysis; fragment ions; mass spectrometry; intact proteins ... See more keywords
Mass Spectrometry of Intact Proteins Reveals +98 u Chemical Artifacts Following Precipitation in Acetone.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.6b00841
Abstract: Protein precipitation in acetone is frequently employed ahead of mass spectrometry for sample preconcentration and purification. Unfortunately, acetone is not chemically inert; mass artifacts have previously been observed on glycine-containing peptides when exposed to acetone… read more here.
Keywords: precipitation; precipitation acetone; mass spectrometry; intact proteins ... See more keywords
Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.9b00071
Abstract: Although thousands of intact proteins have been feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope… read more here.
Keywords: isotope labeling; global quantification; chemical isotope; quantification intact ... See more keywords
In situ mass spectrometry analysis of intact proteins and protein complexes from biological substrates
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Society Transactions"
DOI: 10.1042/bst20190793
Abstract: Advances in sample preparation, ion sources and mass spectrometer technology have enabled the detection and characterisation of intact proteins. The challenges associated include an appropriately soft ionisation event, efficient transmission and detection of the often… read more here.
Keywords: spectrometry; intact proteins; mass spectrometry; analysis ... See more keywords
MALDI HiPLEX-IHC: multiomic and multimodal imaging of targeted intact proteins in tissues
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Chemistry"
DOI: 10.3389/fchem.2023.1182404
Abstract: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is one of the most widely used methods for imaging the spatial distribution of unlabeled small molecules such as metabolites, lipids and drugs in tissues. Recent progress has… read more here.
Keywords: maldi hiplex; hiplex ihc; multiomic multimodal; intact proteins ... See more keywords