Effect of Temporal Expression of Integral Membrane Proteins by Baculovirus Expression Vector System
Sign Up to like & getrecommendations! Published in 2018 at "Molecular Biotechnology"
DOI: 10.1007/s12033-018-0099-y
Abstract: Integral membrane proteins (IMPs) are popular target for drugs, but their resolved structures have been overlooked when compared with cytosolic proteins. The main reason is that IMPs usually need intensive post-translational modifications and they are… read more here.
Keywords: system; membrane proteins; integral membrane; baculovirus expression ... See more keywords
Identification and initial functional characterization of lysosomal integral membrane protein type 2 (LIMP-2) in turbot (Scophthalmus maximus L.).
Sign Up to like & getrecommendations! Published in 2019 at "Developmental and comparative immunology"
DOI: 10.1016/j.dci.2019.103412
Abstract: The immune system protects organism from external pathogens, this progress starts with the pathogen recognition by pattern recognition receptors (PRRs). As a group of PRRs, the class B scavenger receptors showed important roles in phagocytosis.… read more here.
Keywords: protein type; integral membrane; limp; lysosomal integral ... See more keywords
Integral membrane enzymes in eicosanoid metabolism: Structures, mechanisms and inhibitor design.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2020.07.020
Abstract: Eicosanoids are potent lipid mediators involved in central physiological processes such as hemostasis, renal function and parturition. When formed in excess, eicosanoids become critical players in a range of pathological conditions, in particular pain, fever,… read more here.
Keywords: integral membrane; metabolism; enzymes eicosanoid; membrane enzymes ... See more keywords
New crystal forms of the integral membrane Escherichia coli quinol:fumarate reductase suggest that ligands control domain movement.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of structural biology"
DOI: 10.1016/j.jsb.2017.11.004
Abstract: Quinol:fumarate reductase (QFR) is an integral membrane protein and a member of the respiratory Complex II superfamily. Although the structure of Escherichia coli QFR was first reported almost twenty years ago, many open questions of… read more here.
Keywords: integral membrane; coli; fumarate reductase; crystal forms ... See more keywords
Development of a biochemical and biophysical suite for integral membrane protein targets: A review.
Sign Up to like & getrecommendations! Published in 2019 at "Protein expression and purification"
DOI: 10.1016/j.pep.2019.105545
Abstract: The generation of integral membrane proteins (IMPs) in heterologous systems and their characterization remains a major challenge in biomedical research. Significant efforts have been invested both in academia and in the pharmaceutical industry to establish… read more here.
Keywords: integral membrane; biochemical biophysical; biophysical suite; suite integral ... See more keywords
Effects of Esterified Styrene-Maleic Acid Copolymer Degradation on Integral Membrane Protein Extraction.
Sign Up to like & getrecommendations! Published in 2022 at "Biomacromolecules"
DOI: 10.1021/acs.biomac.2c00928
Abstract: The detergent-free extraction of integral membrane proteins using styrene-maleic acid copolymers (SMAs) has shown promise as a potentially effective technique to isolate proteins in a more native-like conformation. As the field continues to develop, the… read more here.
Keywords: integral membrane; styrene maleic; extraction; protein extraction ... See more keywords
Stabilization and structure determination of integral membrane proteins by termini restraining
Sign Up to like & getrecommendations! Published in 2022 at "Nature Protocols"
DOI: 10.1038/s41596-021-00656-5
Abstract: Integral membrane proteins isolated from cellular environment often lose activity and native conformation required for functional analyses and structural studies. Even in their native state, they lack sufficient surfaces to form crystal contacts. Furthermore, most… read more here.
Keywords: integral membrane; membrane; membrane proteins; structure determination ... See more keywords
A sensitive fluorescence-based assay to monitor enzymatic activity of the essential integral membrane protein Apolipoprotein N-acyltransferase (Lnt)
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-52106-8
Abstract: Lipoprotein modification is an essential process in Gram-negative bacteria. The action of three integral membrane proteins that catalyze the transfer of fatty acids derived from membrane phospholipids or cleave the signal peptide of the lipoprotein… read more here.
Keywords: assay monitor; integral membrane; fluorescence based; based assay ... See more keywords
Molecular interactions of the M and E integral membrane proteins of SARS-CoV-2.
Sign Up to like & getrecommendations! Published in 2021 at "Faraday discussions"
DOI: 10.1039/d1fd00031d
Abstract: Specific lipid-protein interactions are key for cellular processes, and even more so for the replication of pathogens. The COVID-19 pandemic has drastically changed our lives and caused the death of nearly four million people worldwide,… read more here.
Keywords: protein; membrane proteins; integral membrane; proteins sars ... See more keywords
Hydrogen/deuterium exchange-mass spectrometry of integral membrane proteins in native-like environments: current scenario and the way forward
Sign Up to like & getrecommendations! Published in 2023 at "Essays in Biochemistry"
DOI: 10.1042/ebc20220173
Abstract: Abstract Integral membrane proteins (IMPs) perform a range of diverse functions and their dysfunction underlies numerous pathological conditions. Consequently, IMPs constitute most drug targets, and the elucidation of their mechanism of action has become an… read more here.
Keywords: integral membrane; native like; membrane proteins; hydrogen deuterium ... See more keywords
Bivalent recognition of fatty acyl-CoA by a human integral membrane palmitoyltransferase
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2022050119
Abstract: Significance Protein palmitoylation is one of the most highly abundant protein modifications, through which long-chain fatty acids get attached to cysteines by a thioester linkage. It plays critically important roles in growth signaling, the organization… read more here.
Keywords: integral membrane; chemistry; palmitoyl coa; coa ... See more keywords