Lymphoblast-derived integration-free iPSC line AD-TREM2-1 from a 67year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant.
Sign Up to like & getrecommendations! Published in 2018 at "Stem cell research"
DOI: 10.1016/j.scr.2018.03.011
Abstract: Human lymphoblast cells from a male diagnosed with Alzheimer's disease (AD) expressing the TREM2 p.R47H variant were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids harbouring OCT4, SOX2, NANOG, LIN28,… read more here.
Keywords: line; expressing trem2; alzheimer disease; r47h variant ... See more keywords
A collection of three integration-free iPSCs derived from old male and female healthy subjects.
Sign Up to like & getrecommendations! Published in 2019 at "Stem cell research"
DOI: 10.1016/j.scr.2019.101663
Abstract: Here, we present the characterization of three iPSC lines derived from dermal fibroblasts of old healthy subjects. Fibroblasts were reprogrammed using Sendai viral vectors encoding OCT4, SOX2, KLF4 and c-MYC. The iPSCs expressed endogenous pluripotency… read more here.
Keywords: free ipscs; ipscs; integration free; healthy subjects ... See more keywords
Generation of stable integration-free pig induced pluripotent stem cells under chemically defined culture condition.
Sign Up to like & getrecommendations! Published in 2023 at "Cell proliferation"
DOI: 10.1111/cpr.13487
Abstract: Genome integration-free pig induced pluripotent stem cells (iPSCs) bring tremendous value in pre-clinical testing of regenerative medicine, as well as conservation and exploitation of endangered or rare local pig idioplasmatic resources. However, due to a… read more here.
Keywords: culture; pig; integration free; ipscs ... See more keywords
Generation of Otic Lineages from Integration-Free Human-Induced Pluripotent Stem Cells Reprogrammed by mRNAs
Sign Up to like & getrecommendations! Published in 2020 at "Stem Cells International"
DOI: 10.1155/2020/3692937
Abstract: Damage to the sensory hair cells and the spiral ganglion neurons of the cochlea leads to deafness. Induced pluripotent stem cells (iPSCs) are a promising tool to regenerate the cells in the inner ear that… read more here.
Keywords: cell; human induced; integration free; pluripotent stem ... See more keywords
Fast-Track and Integration-Free Method of Genome Editing by CRISPR/Cas9 in Murine Pluripotent Stem Cells
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.819906
Abstract: The CRISPR/Cas9 system has unprecedentedly revolutionized genome-editing technology, which is being successfully applied virtually in all branches of biological sciences. Although much success has been attained in gene manipulation, still the majority of methods are… read more here.
Keywords: genome editing; crispr cas9; integration free; pluripotent stem ... See more keywords