Construction of ajmalicine and sanguinarine de novo biosynthetic pathways using stable integration sites in yeast
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.28040
Abstract: Yeast cell factories have been increasingly employed for producing plant‐derived natural products. Unfortunately, the stability of plant natural product biosynthetic pathway genes, particularly when driven by the same sets of promoters and terminators, remains one… read more here.
Keywords: construction; integration sites; integration; stable integration ... See more keywords
Droplet-microfluidics-assisted sequencing of HIV proviruses and their integration sites in cells from people on antiretroviral therapy.
Sign Up to like & getrecommendations! Published in 2022 at "Nature biomedical engineering"
DOI: 10.1038/s41551-022-00864-8
Abstract: The human immunodeficiency virus (HIV) integrates its genome into that of infected cells and may enter an inactive state of reversible latency that cannot be targeted using antiretroviral therapy. Sequencing such a provirus and the… read more here.
Keywords: droplet; cells people; hiv; antiretroviral therapy ... See more keywords
Expansion of EasyClone-MarkerFree toolkit for Saccharomyces cerevisiae genome with new integration sites
Sign Up to like & getrecommendations! Published in 2021 at "FEMS Yeast Research"
DOI: 10.1093/femsyr/foab027
Abstract: ABSTRACT Biotechnological production requires genetically stable recombinant strains. To ensure genomic stability, recombinant DNA is commonly integrated into the genome of the host strain. Multiple genetic tools have been developed for genomic integration into baker's… read more here.
Keywords: saccharomyces cerevisiae; integration; toolkit; easyclone markerfree ... See more keywords
Identification of genome integration sites for developing a CRISPR‐based gene expression toolkit in Yarrowia lipolytica
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14060
Abstract: With the rapid development of synthetic biology, the oleaginous yeast Yarrowia lipolytica has become an attractive microorganism for chemical production. To better optimize and reroute metabolic pathways, we have expanded the CRISPR‐based gene expression toolkit… read more here.
Keywords: integration sites; integration; expression; gene expression ... See more keywords
Ub-ISAP: a streamlined UNIX pipeline for mining unique viral vector integration sites from next generation sequencing data
Sign Up to like & getrecommendations! Published in 2017 at "BMC Bioinformatics"
DOI: 10.1186/s12859-017-1719-4
Abstract: BackgroundThe analysis of viral vector genomic integration sites is an important component in assessing the safety and efficiency of patient treatment using gene therapy. Alongside this clinical application, integration site identification is a key step… read more here.
Keywords: integration sites; viral vector; integration; pipeline ... See more keywords
Detecting virus integration sites based on multiple related sequencing data by VirTect
Sign Up to like & getrecommendations! Published in 2018 at "BMC Medical Genomics"
DOI: 10.1186/s12920-018-0461-8
Abstract: BackgroundSince tumor often has a high level of intra-tumor heterogeneity, multiple tumor samples from the same patient at different locations or different time points are often sequenced to study tumor intra-heterogeneity or tumor evolution. In… read more here.
Keywords: virus integration; integration sites; integration; multiple related ... See more keywords
Discovering HPV16 and HPV18 infection status and cancer gene variations in cervical cancer patient specimens by using Next-generation sequencing.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Clinical Oncology"
DOI: 10.1200/jco.2019.37.15_suppl.e13040
Abstract: e13040 Background: Human papillomavirus (HPV) infection has been reported as an important cause of cervical cancer for many years. HPV16 and HPV18 are the two most prevalent types in cervical cancer5. Integration of HPV DNA… read more here.
Keywords: integration sites; integration; cancer; gene ... See more keywords
Higher-Order Chromatin Structures of Chromosomally Integrated HHV-6A Predict Integration Sites
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Cellular and Infection Microbiology"
DOI: 10.3389/fcimb.2021.612656
Abstract: Human herpesvirus -6A and 6B (HHV-6A/B) can integrate their genomes into the telomeres of human chromosomes. Viral integration can occur in several cell types, including germinal cells, resulting in individuals that harbor the viral genome… read more here.
Keywords: integration; chromatin; higher order; integrated hhv ... See more keywords