Recombinant Production and Intein-Mediated Purification of an Antimicrobial Peptide, BR2
Sign Up to like & getrecommendations! Published in 2017 at "International Journal of Peptide Research and Therapeutics"
DOI: 10.1007/s10989-017-9583-7
Abstract: Nowadays, targeted therapy of cancer is under intensive focus of many investigations due to severe side effects imposed by various cancer chemotherapeutics. BR2 is a modified antimicrobial cell penetrating peptide with confirmed capability of delivering… read more here.
Keywords: peptide; br2; recombinant production; purification ... See more keywords
Pilot production of the recombinant peptide toxin of Heteractis crispa as a potential analgesic by intein-mediated technology.
Sign Up to like & getrecommendations! Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2017.12.011
Abstract: APHC3 is an analgesic polypeptide that was found in the sea anemone (Heteractis crispa), and contains 56 amino acid residues. This polypeptide is of interest for the development of medications for diseases, associated with inflammatory… read more here.
Keywords: peptide; production; heteractis crispa; aphc3 ... See more keywords
Allosteric influence of extremophile hairpin motif mutations on the protein splicing activity of a hyperthermophilic intein.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00348
Abstract: Protein splicing is a post-translational process mediated by an intein, whereby the intein excises itself from a precursor protein with concomitant ligation of the flanking two polypeptides. The intein that interrupts the DNA Polymerase II… read more here.
Keywords: protein splicing; motif; extremophile hairpin; intein ... See more keywords
Structural, Dynamic, and Functional Characterization of a DnaX Mini-intein Derived from Spirulina platensis Provides Important Insights into Intein-Mediated Catalysis of Protein Splicing.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemistry"
DOI: 10.1021/acs.biochem.0c00828
Abstract: Protein splicing is a self-catalyzed post-translational modification in which the intein enzyme excises itself from a precursor protein and ligates the flanking sequences to produce a mature protein. We report the solution structure of a… read more here.
Keywords: protein; dnax mini; mini intein; intein ... See more keywords
A functional interplay between intein and extein sequences in protein splicing compensates for the essential block B histidine† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc01074a
Sign Up to like & getrecommendations! Published in 2019 at "Chemical Science"
DOI: 10.1039/c8sc01074a
Abstract: Steric bulk can compensate for a catalytically critical histidine in an intein's active site and promote the N–S acyl shift. read more here.
Keywords: interplay intein; histidine; extein sequences; functional interplay ... See more keywords
A functional interplay between intein and extein sequences in protein splicing compensates for the essential block B histidine.
Sign Up to like & getrecommendations! Published in 2019 at "Chemical Science"
DOI: 10.2210/pdb6frg/pdb
Abstract: Inteins remove themselves from a precursor protein by protein splicing. Due to the concomitant structural changes of the host protein, this self-processing reaction has enabled many applications in protein biotechnology and chemical biology. We show… read more here.
Keywords: intein; block histidine; extein; protein splicing ... See more keywords
The Convergence of the Hedgehog/Intein Fold in Different Protein Splicing Mechanisms
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21218367
Abstract: Protein splicing catalyzed by inteins utilizes many different combinations of amino-acid types at active sites. Inteins have been classified into three classes based on their characteristic sequences. We investigated the structural basis of the protein… read more here.
Keywords: class; class intein; hedgehog intein; protein splicing ... See more keywords