Development of HBc virus-like particles as modular nanocarrier by intein-mediated trans-splicing.
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2020.10.081
Abstract: Hepatitis B virus core protein (HBc) spontaneously assembles as Virus-like particles (VLPs) in Escherichia coli (E. coli) which is extensively used as a nanocarrier to boost antigen immunogenicity. Genetic fusion of cargo protein with HBc occasionally… read more here.
Keywords: intein mediated; virus like; hbc vlps; hbc ... See more keywords
Reduction of non‐specific toxicity of immunotoxin by intein mediated reconstitution on target cells
Sign Up to like & getrecommendations! Published in 2019 at "International Immunopharmacology"
DOI: 10.1016/j.intimp.2018.11.039
Abstract: &NA; Recombinant immunotoxins are chimeric proteins composed of a targeting peptide that binds to a specific tumor antigen and a toxin protein killing target cells. Recombinant immunotoxin exhibits potent cancer inhibiting effects both in vivo… read more here.
Keywords: specific toxicity; intein mediated; non specific; toxicity ... See more keywords
Cell-Based Biosensors Based on Intein-Mediated Protein Engineering for Detection of Biologically Active Signaling Molecules.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01481
Abstract: Live-cell-based biosensors have emerged as a useful tool for biotechnology and chemical biology. Genetically encoded sensor cells often use bimolecular fluorescence complementation or fluorescence resonance energy transfer to build a reporter unit that suffers from… read more here.
Keywords: protein; intein mediated; active signaling; cell based ... See more keywords
Novel Split Intein-Mediated Enzymatic Channeling Accelerates the Multimeric Bioconversion Pathway of Ginsenoside.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00216
Abstract: Cascade reaction systems, such as protein fusion and synthetic protein scaffold systems, can both spatially control the metabolic flux and boost the productivity of multistep enzymatic reactions. Despite many efforts to generate fusion proteins, this… read more here.
Keywords: system; split; multimeric bioconversion; intein mediated ... See more keywords
Split Intein-Mediated Protein Ligation for detecting protein-protein interactions and their inhibition
Sign Up to like & getrecommendations! Published in 2020 at "Nature Communications"
DOI: 10.1038/s41467-020-16299-1
Abstract: Here, to overcome many limitations accompanying current available methods to detect protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated Protein Ligation (SIMPL). In this approach, bait and prey proteins are respectively… read more here.
Keywords: protein; intein mediated; protein interactions; protein protein ... See more keywords
Liver gene therapy with intein‐mediated F8 trans‐splicing corrects mouse haemophilia A
Sign Up to like & getrecommendations! Published in 2022 at "EMBO Molecular Medicine"
DOI: 10.15252/emmm.202115199
Abstract: Liver gene therapy with adeno‐associated viral (AAV) vectors is under clinical investigation for haemophilia A (HemA), the most common inherited X‐linked bleeding disorder. Major limitations are the large size of the F8 transgene, which makes… read more here.
Keywords: trans splicing; intein mediated; liver gene; gene therapy ... See more keywords
Intein-Mediated Protein Engineering for Cell-Based Biosensors
Sign Up to like & getrecommendations! Published in 2022 at "Biosensors"
DOI: 10.3390/bios12050283
Abstract: Cell-based sensors provide a flexible platform for screening biologically active targets and for monitoring their interactions in live cells. Their applicability extends across a vast array of biological research and clinical applications. Particularly, cell-based sensors… read more here.
Keywords: cell based; cell; mediated protein; protein engineering ... See more keywords
The Inducible Intein-Mediated Self-Cleaving Tag (IIST) System: A Novel Purification and Amidation System for Peptides and Proteins
Sign Up to like & getrecommendations! Published in 2021 at "Molecules"
DOI: 10.3390/molecules26195948
Abstract: An efficient self-cleavable purification tag could be a powerful tool for purifying recombinant proteins and peptides without additional proteolytic processes using specific proteases. Thus, the intein-mediated self-cleavage tag was developed and has been commercially available… read more here.
Keywords: inducible intein; system; iist system; intein mediated ... See more keywords