Coimmunoprecipitation of Interacting Proteins in Plants.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7871-7_19
Abstract: Protein-protein interactions discovered by yeast two-hybrid systems must be confirmed in vivo in a homologous system. In the case of plants, one of the easiest and fastest methods to validate protein interactions in vivo is… read more here.
Keywords: benthamiana leaves; coimmunoprecipitation interacting; coimmunoprecipitation; interacting proteins ... See more keywords
Mass spectrometry-based proteomic analysis of potential infectious bursal disease virus VP3-interacting proteins in chicken embryo fibroblasts cells.
Sign Up to like & getrecommendations! Published in 2021 at "Virus genes"
DOI: 10.1007/s11262-021-01828-x
Abstract: The structural protein VP3 of infectious bursal disease virus (IBDV) plays a critical role in viral assembly, replication, immune escape, and anti-apoptosis. Interaction between VP3 and host protein factors can affect stages in the viral… read more here.
Keywords: vp3; infectious bursal; vp3 interacting; interacting proteins ... See more keywords
Co‐Expression Analysis Reveals Mechanisms Underlying the Varied Roles of NOTCH1 in NSCLC
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Thoracic Oncology"
DOI: 10.1016/j.jtho.2018.10.162
Abstract: Introduction: Notch receptor family dysregulation can be tumor promoting or suppressing depending on cellular context. Our studies shed light on the mechanistic differences that are responsible for NOTCH1’s opposing roles in lung adenocarcinoma and lung… read more here.
Keywords: lung adenocarcinoma; analysis; interacting proteins; expression ... See more keywords
Identification and characterization of Poly(ADP-ribose) polymerase-1 interacting proteins during development of Dictyostelium discoideum.
Sign Up to like & getrecommendations! Published in 2021 at "Protein expression and purification"
DOI: 10.1016/j.pep.2021.105923
Abstract: Poly(ADP-ribose) polymerase-1 (PARP-1) is a multifunctional protein that is associated with various biological processes like chromatin remodeling, DNA damage, cell death etc. In Dictyostelium discoideum, PARP-1 has also been implicated in cellular differentiation and development.… read more here.
Keywords: protein; poly adp; interacting proteins; discoideum ... See more keywords
PACTS-Assisted Thermal Proteome Profiling for Use in Identifying Peptide-Interacting Proteins.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c00581
Abstract: Bioactive peptides play important roles in various biological processes. However, the traditional methods for profiling the peptide-interacting proteins require modifications to the peptide molecules, often leading to false identifications. We found that the interaction between… read more here.
Keywords: thermal proteome; pacts assisted; interacting proteins; peptide interacting ... See more keywords
Proteomic and Biochemical Analyses Reveal a Novel Mechanism for Promoting Protein Ubiquitination and Degradation by UFBP1, a Key Component of Ufmylation.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.7b00843
Abstract: Protein post-translational modification by ubiquitin-fold modifier 1, UFM1, regulates many biological processes such as response to endoplasmic reticulum stress and regulation of tumor progression. A recent study has indicated that the UFM1-binding and PCI domain-containing… read more here.
Keywords: degradation; mechanism; protein ubiquitination; interacting proteins ... See more keywords
Proteomic Investigation of Murine Neuronal α7-Nicotinic Acetylcholine Receptor Interacting Proteins.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.8b00618
Abstract: The α7-nicotinic acetylcholine receptor (α7-nAChR) is a ligand-gated ion channel that is expressed widely in vertebrates and is the principal high-affinity α-bungarotoxin (α-bgtx) binding protein in the mammalian CNS. α7-nAChRs associate with proteins that can… read more here.
Keywords: interacting proteins; acetylcholine receptor; nicotinic acetylcholine; affinity ... See more keywords
Compartmentalization of the proteasome-interacting proteins during sperm capacitation
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-49024-0
Abstract: Ubiquitination is a stable, reversible posttranslational modification of target proteins by covalent ligation of the small chaperone protein ubiquitin. Most commonly ubiquitination targets proteins for degradation/recycling by the 26S proteasome in a well-characterized enzymatic cascade.… read more here.
Keywords: sperm capacitation; proteasome interacting; capacitation; interacting proteins ... See more keywords
Methylated-antibody affinity purification to improve proteomic identification of plant RNA polymerase Pol V complex and the interacting proteins
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/srep42943
Abstract: Affinity purification followed by enzymatic digestion and mass spectrometry has been widely utilized for the sensitive detection of interacting proteins and protein complexes in various organisms. In plants, the method is technically challenging due to… read more here.
Keywords: identification; interacting proteins; affinity purification; pol complex ... See more keywords
Nsp2 has the potential to be a drug target revealed by global identification of SARS-CoV-2 Nsp2-interacting proteins.
Sign Up to like & getrecommendations! Published in 2021 at "Acta biochimica et biophysica Sinica"
DOI: 10.1093/abbs/gmab088
Abstract: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global health threat since December 2019, and there is still no highly effective drug to control the pandemic. To facilitate drug target identification for drug development,… read more here.
Keywords: nsp2 interacting; drug; interacting proteins; drug target ... See more keywords
Microtubule plus-end tracking proteins: novel modulators of cardiac sodium channels and arrhythmogenesis.
Sign Up to like & getrecommendations! Published in 2023 at "Cardiovascular research"
DOI: 10.1093/cvr/cvad052
Abstract: The cardiac sodium channel NaV1.5 is an essential modulator of cardiac excitability, with decreased NaV1.5 levels at the plasma membrane and consequent reduction in sodium current (INa) leading to potentially lethal cardiac arrhythmias. NaV1.5 is… read more here.
Keywords: intercalated disc; sodium; interacting proteins; membrane ... See more keywords