Ultrasensitive Firefly Luminescent Intermediate-Based Protein-Protein Interaction Assay (FlimPIA) Based on the Functional Complementation of Mutant Firefly Luciferases.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6940-1_8
Abstract: We recently developed a protein-protein interaction assay, FlimPIA (Firefly luminescent intermediate-based Protein-protein Interaction Assay) based on the catalytic mechanism of firefly luciferase (Fluc) that can be divided into two half-reactions: the adenylation step and the… read more here.
Keywords: protein; protein interaction; firefly; protein protein ... See more keywords
DNA covalently linked to graphene oxide for biotin-streptavidin interaction assay.
Sign Up to like & getrecommendations! Published in 2017 at "Talanta"
DOI: 10.1016/j.talanta.2016.10.096
Abstract: Based on terminal protection of small molecule-linked DNA and the covalently linking DNA to graphene oxide (GO) strategy, a high resisting nonspecific probe displacement platform for small molecule-protein interaction assay is proposed in this work.… read more here.
Keywords: dna; dna covalently; small molecule; interaction ... See more keywords
In Cellulo Protein-mRNA Interaction Assay to Determine the Action of G-Quadruplex-Binding Molecules
Sign Up to like & getrecommendations! Published in 2018 at "Molecules"
DOI: 10.3390/molecules23123124
Abstract: Protein-RNA interactions (PRIs) control pivotal steps in RNA biogenesis, regulate multiple physiological and pathological cellular networks, and are emerging as important drug targets. However, targeting of specific protein-RNA interactions for therapeutic developments is still poorly… read more here.
Keywords: protein mrna; mrna interaction; cellulo protein; molecules cellulo ... See more keywords