Mechanistic modeling based process analytical technology implementation for pooling in hydrophobic interaction chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology Progress"
DOI: 10.1002/btpr.2758
Abstract: A major challenge in chromatography purification of therapeutic proteins is batch‐to‐batch variability with respect to impurity levels and product concentration in the feed. Mechanistic model can enable process analytical technology (PAT) implementation by predicting impact… read more here.
Keywords: chromatography; hydrophobic interaction; analytical technology; model ... See more keywords
Considerations for operational space definition and optimization of a no-salt flowthrough hydrophobic interaction chromatography purification step.
Sign Up to like & getrecommendations! Published in 2023 at "Biotechnology progress"
DOI: 10.1002/btpr.3351
Abstract: No-salt flowthrough hydrophobic interaction chromatography (HIC) has been shown to effectively remove process and product-related impurities from bioprocess streams. In this publication, a panel of six antibodies has been used to demonstrate operating principles for… read more here.
Keywords: salt flowthrough; flowthrough hydrophobic; interaction chromatography; hydrophobic interaction ... See more keywords
Quantitation of antibiotics in fresh fermentation medium by hydrophilic interaction chromatography mass spectrometry
Sign Up to like & getrecommendations! Published in 2025 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-025-05775-6
Abstract: This study presents the development of a sophisticated liquid chromatography-mass spectrometry approach leveraging hydrophilic interaction chromatography (HILIC) for the quantification of kanamycin and spectinomycin in fermentation media. The method was validated per International Council for… read more here.
Keywords: interaction chromatography; mass spectrometry; chromatography mass; hydrophilic interaction ... See more keywords
Refolding with Simultaneous Purification of Recombinant Core Streptavidin Using Single-step High-performance Hydrophobic Interaction Chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-019-0041-6
Abstract: Streptavidin has applied to many areas including detection, purification, labeling, crosslinking and immobilization resulting in a high demand on its production. In this study, we report a method for preparation of recombinant core streptavidin (cSAV)… read more here.
Keywords: chromatography; hydrophobic interaction; recombinant core; streptavidin ... See more keywords
Characterization and quantification of succinimide using peptide mapping under low-pH conditions and hydrophobic interaction chromatography.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2018.11.021
Abstract: Characterization of asparagine deamidation and aspartic acid isomerization is an important aspect of biotherapeutic protein analysis due to the potential negative effect of these modifications on drug efficacy and stability. Succinimide has long been known… read more here.
Keywords: hydrophobic interaction; mapping low; interaction chromatography; peptide mapping ... See more keywords
Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.08.051
Abstract: The separation of some zwitterionic, basic and neutral antibiotic and antiretroviral compounds was studied using hydrophilic interaction chromatography (HILIC) on bare silica, bonded amide and urea superficially porous phases. The differences in the selectivity and… read more here.
Keywords: partial equilibration; equilibration; elution; hydrophilic interaction ... See more keywords
Isolation and quantitation of exosomes isolated from human plasma via hydrophobic interaction chromatography using a polyester, capillary-channeled polymer fiber phase.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.07.035
Abstract: Exosomes are one class of extracellular vesicles (30-150 nm diameter) that are secreted by cells. These small vesicles hold a great deal of promise in disease diagnostics, as they display the same protein biomarkers as their… read more here.
Keywords: interaction chromatography; channeled polymer; isolation; hydrophobic interaction ... See more keywords
Rapid separation of blood plasma exosomes from low-density lipoproteins via a hydrophobic interaction chromatography method on a polyester capillary-channeled polymer fiber phase.
Sign Up to like & getrecommendations! Published in 2021 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2021.338578
Abstract: Exosomes are membrane-bound, cell-secreted vesicles, with sizes ranging from 30 to 150 nm. Exosomes in blood plasma have become proposed targets as measurable indicators of disease conditions. Current methods for plasma-based exosome isolation are time-consuming, complex,… read more here.
Keywords: hydrophobic interaction; plasma; low density; interaction chromatography ... See more keywords
Nanoparticle-doped polystyrene/polyacrylonitrile nanofiber membrane with hierarchical structure as promising protein hydrophobic interaction chromatography media
Sign Up to like & getrecommendations! Published in 2019 at "Composites Communications"
DOI: 10.1016/j.coco.2019.08.008
Abstract: Abstract Hydrophobic interaction chromatography (HIC) as an indispensable method for protein purification has attracted considerable attentions of researchers as well as biopharmaceutical industries. However, the operation pressure-drop and flow rate of the typical HIC porous… read more here.
Keywords: polystyrene polyacrylonitrile; interaction chromatography; hydrophobic interaction;
Glutathione-modified ordered mesoporous silicas for enrichment of N-linked glycopeptides by hydrophilic interaction chromatography.
Sign Up to like & getrecommendations! Published in 2020 at "Talanta"
DOI: 10.1016/j.talanta.2020.121082
Abstract: Hydrophilic interaction chromatography (HILIC), as one of the important methods for glycopeptides enrichment, has attracted the attention of more and more researchers due to the high efficiency in glycopeptides enrichment. Ordered mesoporous silica materials have… read more here.
Keywords: mesoporous silicas; hydrophilic interaction; linked glycopeptides; interaction chromatography ... See more keywords
Selectivity and Resolving Power of Hydrophobic Interaction Chromatography Targeting the Separation of Monoclonal Antibody Variants
Sign Up to like & getrecommendations! Published in 2024 at "Analytical Chemistry"
DOI: 10.1021/acs.analchem.3c04011
Abstract: This study presents a comprehensive investigation of the mechanistic understanding of retention and selectivity in hydrophobic interaction chromatography. It provides valuable insights into crucial method-development parameters involved in achieving chromatographic resolution for profiling molecular variants… read more here.
Keywords: interaction chromatography; hydrophobic interaction; selectivity; selectivity resolving ... See more keywords