Hydrophilic interaction liquid chromatography-mass spectrometry as a new tool for the characterization of intact semi-synthetic glycoproteins.
Sign Up to like & getrecommendations! Published in 2017 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2017.05.020
Abstract: Improved methods for detailed characterization of complex glycoproteins are required in the growing sector of biopharmaceuticals. Hydrophilic interaction liquid chromatography (HILIC) coupled to high resolution (HR) time-of-flight mass spectrometric (TOF-MS) detection was examined for the… read more here.
Keywords: characterization intact; interaction liquid; semi synthetic; hydrophilic interaction ... See more keywords
Hydrophilic interaction liquid chromatography of aminoglycoside antibiotics with a diol-type stationary phase.
Sign Up to like & getrecommendations! Published in 2018 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2018.08.008
Abstract: Owing to their pronounced polarity, hydrophilic interaction liquid chromatography (HILIC) can be considered as the elective choice for the LC analysis of aminoglycoside (AG) antibiotics. In the present work, a gradient program was optimized for… read more here.
Keywords: type stationary; diol type; interaction liquid; stationary phase ... See more keywords
Towards a high peak capacity of 130 using nanoflow hydrophilic interaction liquid chromatography.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.01.060
Abstract: Hydrophilic interaction liquid chromatography, HILIC, is a relatively new HPLC mode. Compared with other HPLC modes, HILIC is a high resolution chromatographic mode with high peak capacity for separations of complex mixtures. Although the separation… read more here.
Keywords: chromatography; high peak; interaction liquid; hydrophilic interaction ... See more keywords
First profiling in hydrophilic interaction liquid chromatography of intact human chorionic gonadotropin isoforms.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2019.06.014
Abstract: The study of glycoproteins is a rapidly growing field, which is not surprising considering that approximately 70% of human proteins are glycosylated and that numerous biological functions are associated to the glycosylation. In this work,… read more here.
Keywords: interaction liquid; hcg; hydrophilic interaction; human chorionic ... See more keywords
A novel hydrophilic interaction liquid chromatography method for the analysis of arterolane; Isolation and structural elucidation of its major degradation product by LC-HRMS and NMR.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2020.113279
Abstract: Adequate retention of arterolane (ART) has not been published in the literature till date; the hydrophilic interaction liquid chromatographic (HILIC) method with improved retention and separation of arterolane from its degradation products are reporting here… read more here.
Keywords: degradation; interaction liquid; method; hydrophilic interaction ... See more keywords
Analysis of highly polar marine biotoxins in seawater by hydrophilic interaction liquid chromatography coupled to high resolution mass spectrometry
Sign Up to like & getrecommendations! Published in 2021 at "MethodsX"
DOI: 10.1016/j.mex.2021.101370
Abstract: The monitoring of marine biotoxins (MBTs) in seawater is presented as an alternative strategy to determine their presence and the possible implications in the ecosystem. For this, an analytical method based on hydrophilic interaction liquid… read more here.
Keywords: marine biotoxins; liquid; interaction liquid; hydrophilic interaction ... See more keywords
Protocol to compare column performance applied to hydrophilic interaction liquid chromatography
Sign Up to like & getrecommendations! Published in 2019 at "Microchemical Journal"
DOI: 10.1016/j.microc.2019.103973
Abstract: Abstract The lack of retention of highly polar solutes in liquid chromatography with reversed phase columns (RPLC) can be solved through the use of the hydrophilic interaction liquid chromatographic (HILIC) mode. Due to the complexity… read more here.
Keywords: performance; interaction liquid; hydrophilic interaction; liquid chromatography ... See more keywords
Hydrophilic Interaction Liquid Chromatography-Mass Spectrometry Characterization of Gangliosides in Biological Samples.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b03523
Abstract: The hydrophilic interaction liquid chromatography (HILIC) coupled to a negative-ion electrospray ionization tandem mass spectrometry (ESI-MS/MS) method has been developed for the identification of a wide range of gangliosides in biological samples. Gangliosides consist of… read more here.
Keywords: mass spectrometry; interaction liquid; hydrophilic interaction; biological samples ... See more keywords
Two-Dimensional MoS2-Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b00461
Abstract: Mass spectrometry (MS)-based glycoproteomics research requires highly efficient sample preparation to eliminate interference from non-glycopeptides and to improve the efficiency of glycopeptide detection. In this work, a novel MoS2/Au-NP (gold nanoparticle)-L-cysteine nanocomposite was prepared for… read more here.
Keywords: enrichment; interaction liquid; two dimensional; hydrophilic interaction ... See more keywords
Low-Bleed Silica-Based Stationary Phase for Hydrophilic Interaction Liquid Chromatography.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01796
Abstract: Bleeding of hydrophilic interaction liquid chromatographic (HILIC) phases is a major problem. A hydrophobic silica surface exhibits low bleeding but does not behave as a HILIC phase. A hydrophilic coating, such as that of poly(vinyl… read more here.
Keywords: phase; low bleed; phase hydrophilic; stationary phase ... See more keywords
A sulfonated chitooligosaccharide modified silica material for hydrophilic interaction liquid chromatography and its chromatographic evaluation
Sign Up to like & getrecommendations! Published in 2018 at "Analytical Methods"
DOI: 10.1039/c8ay00070k
Abstract: A novel stationary phase derived from a sulfonated chitooligosaccharide (COS) was prepared and evaluated in hydrophilic interaction liquid chromatography (HILIC) mode. Fourier-transform infrared spectroscopy (FT-IR), elemental analysis (EA), and thermogravimetric analysis (TGA) supported the successful… read more here.
Keywords: phase; stationary phase; interaction liquid; hydrophilic interaction ... See more keywords