Accurate determination of drug-to-antibody ratio of interchain cysteine-linked antibody–drug conjugates by LC-HRMS
Sign Up to like & getrecommendations! Published in 2019 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-019-02280-5
Abstract: Accurate determination of the drug-to-antibody ratio (DAR) of interchain cysteine-linked antibody–drug conjugates (ADCs) is challenging. High-resolution mass spectrometry (HRMS) analysis of the ADCs at the intact or subunit level provides a feasible way to measure… read more here.
Keywords: dar; cysteine linked; accurate determination; drug ... See more keywords
Structural characterization of a monomethylauristatin-E based ADC that contains 8 drugs conjugated at interchain cysteine residues.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of pharmaceutical and biomedical analysis"
DOI: 10.1016/j.jpba.2021.114309
Abstract: Antibody-drug conjugates (ADCs) with a drug-to-antibody ratio (DAR) of 8 are attractive as therapeutic anti-cancer agents due to the higher levels of cytotoxic payload delivered to tumors. Biophysical characterization of a DAR 8 ADC fully… read more here.
Keywords: dar adc; conjugated interchain; cysteine residues; stability ... See more keywords