Intramolecular and Intermolecular FRET Sensors for GPCRs - Monitoring Conformational Changes and Beyond.
Sign Up to like & getrecommendations! Published in 2018 at "Trends in pharmacological sciences"
DOI: 10.1016/j.tips.2017.10.011
Abstract: Within the past decade, a large increase in structural knowledge from crystallographic studies has significantly fostered our understanding of the structural biology of G protein-coupled receptors (GPCRs). However, information on dynamic events upon receptor activation… read more here.
Keywords: monitoring conformational; gpcrs monitoring; intermolecular fret; sensors gpcrs ... See more keywords
An intermolecular FRET sensor detects the dynamics of T cell receptor clustering
Sign Up to like & getrecommendations! Published in 2017 at "Nature Communications"
DOI: 10.1038/ncomms15100
Abstract: Clustering of the T-cell receptor (TCR) is thought to initiate downstream signalling. However, the detection of protein clustering with high spatial and temporal resolution remains challenging. Here we establish a Förster resonance energy transfer (FRET)… read more here.
Keywords: cell receptor; intermolecular fret; sensor detects; sensor ... See more keywords
Robust evaluation of intermolecular FRET using a large Stokes shift fluorophore as a donor.
Sign Up to like & getrecommendations! Published in 2018 at "BioTechniques"
DOI: 10.2144/btn-2018-0041
Abstract: Fluorescence (or Förster) resonance energy transfer (FRET) is a straightforward and sensitive technique to evaluate molecular interactions. However, most of the popular FRET pairs suffer cross-excitation of the acceptor, which could lead to false positives.… read more here.
Keywords: large stokes; robust evaluation; intermolecular fret; evaluation intermolecular ... See more keywords