Engineered Matrices Enable the Culture of Human Patient‐Derived Intestinal Organoids
Sign Up to like & getrecommendations! Published in 2021 at "Advanced Science"
DOI: 10.1002/advs.202004705
Abstract: Abstract Human intestinal organoids from primary human tissues have the potential to revolutionize personalized medicine and preclinical gastrointestinal disease models. A tunable, fully defined, designer matrix, termed hyaluronan elastin‐like protein (HELP) is reported, which enables… read more here.
Keywords: help materials; engineered matrices; intestinal organoids; help ... See more keywords
A novel morphometry system automatically assessing the growth and regeneration of intestinal organoids.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2018.10.181
Abstract: As compared with 2D cell line cultures, 3D intestinal organoids are better at maximally recapitulating the physiological features of stem cells in vivo. However, the complex 3D structure is an obstacle which must be objectively and… read more here.
Keywords: intestinal organoids; growth; regeneration; morphometry system ... See more keywords
Dynamic Formation of Microvillus Inclusions During Enterocyte Differentiation in Munc18-2–Deficient Intestinal Organoids
Sign Up to like & getrecommendations! Published in 2018 at "Cellular and Molecular Gastroenterology and Hepatology"
DOI: 10.1016/j.jcmgh.2018.08.001
Abstract: Background & Aims Microvillus inclusion disease (MVID) is a congenital intestinal malabsorption disorder caused by defective apical vesicular transport. Existing cellular models do not fully recapitulate this heterogeneous pathology. The aim of this study was… read more here.
Keywords: microvillus inclusions; microscopy; formation; pathology ... See more keywords
Using Microfluidics to Model Mucus
Sign Up to like & getrecommendations! Published in 2020 at "Cellular and Molecular Gastroenterology and Hepatology"
DOI: 10.1016/j.jcmgh.2019.12.003
Abstract: uch of our understanding of molecular mechaMnisms of diseases has relied historically on work performed in cancer cell lines. Although cancer-derived cell lines offer many advantages such as being easy to grow, relatively inexpensive, and… read more here.
Keywords: cell; cell lines; mucus bilayer; mucus ... See more keywords
Tissue Responses to Shiga Toxin in Human Intestinal Organoids
Sign Up to like & getrecommendations! Published in 2020 at "Cellular and Molecular Gastroenterology and Hepatology"
DOI: 10.1016/j.jcmgh.2020.02.006
Abstract: Background & Aims Shiga toxin (Stx)-producing Escherichia coli (eg, O157:H7) infection produces bloody diarrhea, while Stx inhibits protein synthesis and causes the life-threatening systemic complication of hemolytic uremic syndrome. The murine intestinal tract is resistant… read more here.
Keywords: tissue; tissue responses; shiga toxin; human intestinal ... See more keywords
Aspirin Rescues Wnt-Driven Stem-like Phenotype in Human Intestinal Organoids and Increases the Wnt Antagonist Dickkopf-1
Sign Up to like & getrecommendations! Published in 2020 at "Cellular and Molecular Gastroenterology and Hepatology"
DOI: 10.1016/j.jcmgh.2020.09.010
Abstract: Background & Aims Aspirin reduces colorectal cancer (CRC) incidence and mortality. Understanding the biology responsible for this protective effect is key to developing biomarker-led approaches for rational clinical use. Wnt signaling drives CRC development from… read more here.
Keywords: cell; aspirin; wnt driven; stem like ... See more keywords
Drug Inhibition of SARS-CoV-2 Replication in Human Pluripotent Stem Cell–Derived Intestinal Organoids
Sign Up to like & getrecommendations! Published in 2020 at "Cellular and Molecular Gastroenterology and Hepatology"
DOI: 10.1016/j.jcmgh.2020.11.003
Abstract: Background and aims The COVID-19 pandemic has spread worldwide and poses a severe health risk. While most patients present mild symptoms, descending pneumonia can lead to severe respiratory insufficiency. Up to 50% of patients show… read more here.
Keywords: psc hios; drug; pluripotent stem; sars cov ... See more keywords
Transplantation of human intestinal organoids into the mouse mesentery: A more physiologic and anatomic engraftment site
Sign Up to like & getrecommendations! Published in 2018 at "Surgery"
DOI: 10.1016/j.surg.2018.04.048
Abstract: Background: We previously described the development of human intestinal organoids from pluripotent stem cells, as well as their in vivo maturation when transplanted into the mouse kidney capsule. While sufficient for certain aspects of study,… read more here.
Keywords: human intestinal; mouse mesentery; intestinal organoids; transplantation human ... See more keywords
Infection of bat and human intestinal organoids by SARS-CoV-2
Sign Up to like & getrecommendations! Published in 2020 at "Nature Medicine"
DOI: 10.1038/s41591-020-0912-6
Abstract: A novel coronavirus—severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—emerged in humans in Wuhan, China, in December 2019 and has since disseminated globally 1 , 2 . As of April 16, 2020, the confirmed case count… read more here.
Keywords: infection; bat human; human intestinal; sars cov ... See more keywords
Human intestinal organoids express histo-blood group antigens, bind norovirus VLPs, and support limited norovirus replication
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-12736-2
Abstract: Through pluripotent stem cell (PSC) technology, human intestinal organoids (HIOs) with remarkably similarity to the fetal intestine in cellular composition, architecture, and absorptive/secretory functions have been successfully developed, providing a useful in vitro model system… read more here.
Keywords: organoids express; replication human; human intestinal; intestinal organoids ... See more keywords
Culture and differentiation of rabbit intestinal organoids and organoid-derived cell monolayers
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DOI: 10.1038/s41598-021-84774-w
Abstract: Organoids emulate many aspects of their parental tissue and are therefore used to study pathogen-host interactions and other complex biological processes. Here, we report a robust protocol for the isolation, maintenance and differentiation of rabbit… read more here.
Keywords: cell; organoids organoid; differentiation rabbit; rabbit ... See more keywords