Intracellular protein crystallization in living insect cells
Sign Up to like & getrecommendations! Published in 2025 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.70020
Abstract: Crystallization of recombinant proteins in living cells is an emerging approach complementing conventional crystallization techniques. Homogeneous microcrystals well suited for serial diffraction experiments at X‐ray free‐electron lasers and synchrotron sources can be produced in a… read more here.
Keywords: protein crystallization; intracellular protein; crystallization; living insect ... See more keywords
Simple and Efficient Targeted Intracellular Protein Delivery with Self‐Assembled Nanovehicles for Effective Cancer Therapy
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Functional Materials"
DOI: 10.1002/adfm.201906187
Abstract: Protein therapy offers promising prospects for the treatment of various important diseases, thus it is highly desirable to develop a robust carrier that can deliver active proteins into cells. The development of a novel protein… read more here.
Keywords: protein delivery; cdeh nanovehicles; self; intracellular protein ... See more keywords
Intracellular Protein Photoactivation Using Sterically Bulky Caging
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200476
Abstract: Methods for intracellular protein photoactivation have been studied to elucidate the spatial and temporal roles of proteins of interest. In this study, an intracellular protein photoactivation method was developed using sterically bulky caging. The protein… read more here.
Keywords: using sterically; photoactivation; intracellular protein; protein photoactivation ... See more keywords
Enhanced Intracellular Protein Activity by Caveolae-Mediated Endocytosis of Conjugated Polymer/Protein Complexes.
Sign Up to like & getrecommendations! Published in 2025 at "Macromolecular bioscience"
DOI: 10.1002/mabi.202400557
Abstract: Conjugated polymers (CPs) with rigid hydrophobic backbones and polar side chains are known for their efficient cellular entry using various endocytic pathways. Here, the efficient delivery of ribonuclease A (RNase A) into the cytosol of… read more here.
Keywords: intracellular protein; enhanced intracellular; protein activity; activity caveolae ... See more keywords
Intracellular protein complexes involved in synapse assembly in presynaptic neurons.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in protein chemistry and structural biology"
DOI: 10.1016/bs.apcsb.2018.11.008
Abstract: The presynaptic active zone, composed of evolutionarily conserved protein complexes, is a specialized area that serves to orchestrate precise and efficient neurotransmitter release by organizing various presynaptic proteins involved in mediating docking and priming of… read more here.
Keywords: protein complexes; involved synapse; active zone; intracellular protein ... See more keywords
Fibroin particle-supported cationic lipid layers for highly efficient intracellular protein delivery.
Sign Up to like & getrecommendations! Published in 2017 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2017.01.019
Abstract: Directly delivering therapeutic proteins into cells has promise as an intervention without side effects for protein deficiencies caused by genetic defects. However, as negatively charged macromolecules, proteins require carriers for achieving cellular uptake and maintaining… read more here.
Keywords: protein; cationic lipid; protein delivery; intracellular protein ... See more keywords
Interaction of the minocycline with extracelluar protein and intracellular protein by multi-spectral techniques and molecular docking
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Molecular Structure"
DOI: 10.1016/j.molstruc.2016.10.046
Abstract: Abstract The interaction of minocyeline (MNC) with extracelluar protein (lysozyme, LYSO) or intracellular protein (bovine hemoglobin, BHb) was investigated using multi-spectral techniques and molecular docking in vitro. Fluorescence studies suggested that MNC quenched LYSO/BHb fluorescence in… read more here.
Keywords: protein; multi spectral; intracellular protein; extracelluar protein ... See more keywords
Programming of Extra- and Intracellular Protein Coronation through Thiol-Independent Ligand Engineering.
Sign Up to like & getrecommendations! Published in 2025 at "Nano letters"
DOI: 10.1021/acs.nanolett.5c03113
Abstract: Variation in the distal end of ligands can impact biological functions, but the effects of the proximal end of the ligand (PEL) are less clear. This study presents poly(ethylene glycol) (PEG) ligands that bind to… read more here.
Keywords: intracellular protein; programming extra; extra intracellular; protein coronation ... See more keywords
Translocation of an Intracellular Protein via Peptide-Directed Ligation.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.6b01013
Abstract: Ligand-directed reactions allow chemical transformations at very low reactant concentrations and can thus provide access to efficient approaches for the post-translational modification of proteins. The development of these proximity-induced reactions is hampered by the number… read more here.
Keywords: protein; peptide; intracellular protein; protein via ... See more keywords
In-Cell Mass Spectrometry and Ultraviolet Photodissociation Navigates the Intracellular Protein Heterogeneity.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.4c16376
Abstract: Directly probing the heterogeneous conformations of intracellular proteins within their native cellular environment remains a significant challenge in mass spectrometry (MS). Here, we establish an in-cell MS and ultraviolet photodissociation (UVPD) strategy that directly ejects… read more here.
Keywords: intracellular protein; mass spectrometry; ultraviolet photodissociation; mass ... See more keywords
In vitro tracking and intracellular protein distribution in immunology
Sign Up to like & getrecommendations! Published in 2017 at "Immunology and Cell Biology"
DOI: 10.1038/icb.2017.29
Abstract: New imaging techniques have enabled major advances in understanding how immune reactions are initiated, coordinated and controlled. Imaging methods, which were previously mostly descriptive and supplementary to more quantitative approaches, have now reached sufficient precision… read more here.
Keywords: distribution immunology; intracellular protein; tracking intracellular; protein distribution ... See more keywords