Simple and Efficient Targeted Intracellular Protein Delivery with Self‐Assembled Nanovehicles for Effective Cancer Therapy
Sign Up to like & getrecommendations! Published in 2019 at "Advanced Functional Materials"
DOI: 10.1002/adfm.201906187
Abstract: Protein therapy offers promising prospects for the treatment of various important diseases, thus it is highly desirable to develop a robust carrier that can deliver active proteins into cells. The development of a novel protein… read more here.
Keywords: protein delivery; cdeh nanovehicles; self; intracellular protein ... See more keywords
Intracellular Protein Photoactivation Using Sterically Bulky Caging
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200476
Abstract: Methods for intracellular protein photoactivation have been studied to elucidate the spatial and temporal roles of proteins of interest. In this study, an intracellular protein photoactivation method was developed using sterically bulky caging. The protein… read more here.
Keywords: using sterically; photoactivation; intracellular protein; protein photoactivation ... See more keywords
Intracellular protein complexes involved in synapse assembly in presynaptic neurons.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in protein chemistry and structural biology"
DOI: 10.1016/bs.apcsb.2018.11.008
Abstract: The presynaptic active zone, composed of evolutionarily conserved protein complexes, is a specialized area that serves to orchestrate precise and efficient neurotransmitter release by organizing various presynaptic proteins involved in mediating docking and priming of… read more here.
Keywords: protein complexes; involved synapse; active zone; intracellular protein ... See more keywords
Fibroin particle-supported cationic lipid layers for highly efficient intracellular protein delivery.
Sign Up to like & getrecommendations! Published in 2017 at "Biomaterials"
DOI: 10.1016/j.biomaterials.2017.01.019
Abstract: Directly delivering therapeutic proteins into cells has promise as an intervention without side effects for protein deficiencies caused by genetic defects. However, as negatively charged macromolecules, proteins require carriers for achieving cellular uptake and maintaining… read more here.
Keywords: protein; cationic lipid; protein delivery; intracellular protein ... See more keywords
Interaction of the minocycline with extracelluar protein and intracellular protein by multi-spectral techniques and molecular docking
Sign Up to like & getrecommendations! Published in 2017 at "Journal of Molecular Structure"
DOI: 10.1016/j.molstruc.2016.10.046
Abstract: Abstract The interaction of minocyeline (MNC) with extracelluar protein (lysozyme, LYSO) or intracellular protein (bovine hemoglobin, BHb) was investigated using multi-spectral techniques and molecular docking in vitro. Fluorescence studies suggested that MNC quenched LYSO/BHb fluorescence in… read more here.
Keywords: protein; multi spectral; intracellular protein; extracelluar protein ... See more keywords
Translocation of an Intracellular Protein via Peptide-Directed Ligation.
Sign Up to like & getrecommendations! Published in 2017 at "ACS chemical biology"
DOI: 10.1021/acschembio.6b01013
Abstract: Ligand-directed reactions allow chemical transformations at very low reactant concentrations and can thus provide access to efficient approaches for the post-translational modification of proteins. The development of these proximity-induced reactions is hampered by the number… read more here.
Keywords: protein; peptide; intracellular protein; protein via ... See more keywords
In vitro tracking and intracellular protein distribution in immunology
Sign Up to like & getrecommendations! Published in 2017 at "Immunology and Cell Biology"
DOI: 10.1038/icb.2017.29
Abstract: New imaging techniques have enabled major advances in understanding how immune reactions are initiated, coordinated and controlled. Imaging methods, which were previously mostly descriptive and supplementary to more quantitative approaches, have now reached sufficient precision… read more here.
Keywords: distribution immunology; intracellular protein; tracking intracellular; protein distribution ... See more keywords
Amphipathic poly-β-peptides for intracellular protein delivery.
Sign Up to like & getrecommendations! Published in 2022 at "Chemical communications"
DOI: 10.1039/d2cc00453d
Abstract: A series of amphipathic poly-β-peptides are designed for intracellular protein delivery. The poly-β-peptides with higher molecular weight and hydrophobic contents exhibit higher protein loading and superior delivery efficiency. The lead material efficiently delivers proteins into… read more here.
Keywords: protein delivery; amphipathic poly; intracellular protein; poly peptides ... See more keywords
Avoiding entry into intracellular protein degradation pathways by signal mutations increases protein secretion in Pichia pastoris
Sign Up to like & getrecommendations! Published in 2022 at "Microbial Biotechnology"
DOI: 10.1111/1751-7915.14061
Abstract: In our previous study, we serendipitously discovered that protein secretion in the methylotrophic yeast Pichia pastoris is enhanced by a mutation (V50A) in the mating factor alpha (MFα) prepro‐leader signal derived from Saccharomyces cerevisiae. In… read more here.
Keywords: pichia pastoris; degradation pathways; intracellular protein; protein degradation ... See more keywords
Pore forming–mediated intracellular protein delivery for enhanced cancer immunotherapy
Sign Up to like & getrecommendations! Published in 2022 at "Science Advances"
DOI: 10.1126/sciadv.abq4659
Abstract: Directly delivering therapeutic proteins to their intracellular targets remains a great challenge. Here, we apply CD8+ T cells to form pores on the tumor cells’ plasma membranes, enabling perfusion of ribonuclease A (RNase A) and… read more here.
Keywords: pore forming; protein delivery; mediated intracellular; forming mediated ... See more keywords
Physiological Functions of Intracellular Protein Degradation.
Sign Up to like & getrecommendations! Published in 2022 at "Annual review of cell and developmental biology"
DOI: 10.1146/annurev-cellbio-120420-091943
Abstract: While cellular proteins were initially thought to be stable, research over the last decades has firmly established that intracellular protein degradation is an active and highly regulated process: Lysosomal, proteasomal, and mitochondrial degradation systems were… read more here.
Keywords: physiological functions; intracellular protein; protein degradation; degradation ... See more keywords