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Published in 2019 at "Biotechnology advances"
DOI: 10.1016/j.biotechadv.2019.01.002
Abstract: Inulin, a natural fructan, cannot be hydrolyzed by digestive enzymes in the human body and plays a role as a dietary fiber and prebiotic. Due to its versatile physicochemical properties and physiological functions, inulin has…
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Keywords:
molecular modifications;
inulosucrase;
characteristics structural;
features molecular ... See more keywords
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Published in 2019 at "Carbohydrate polymers"
DOI: 10.1016/j.carbpol.2018.12.078
Abstract: Inulosucrase (E.C. 2.4.1.9) is a bacterial fructosyltransferase that synthesizes inulin-type fructooligosaccharide, using sucrose as a substrate. We modulated the size of fructooligosaccharide synthesized by Lactobacillus reuteri 121 inulosucrase using rational designed mutagenesis. Nine residues: D478,…
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Keywords:
inulosucrase;
lactobacillus reuteri;
product;
121 inulosucrase ... See more keywords