Mapping Transposon Insertion Sites by Inverse Polymerase Chain Reaction and Sanger Sequencing.
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DOI: 10.1101/pdb.prot108197
Abstract: Inverse polymerase chain reaction (PCR) is a method designed to amplify a segment of DNA for which only a portion of the sequence is known. The method consists of circularizing the DNA fragment by self-ligation… read more here.
Keywords: transposon insertion; chain reaction; polymerase chain; inverse polymerase ... See more keywords