Impact of Inverted Terminal Repeat Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System
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DOI: 10.1089/hgtb.2016.133
Abstract: Adeno-associated virus (AAV) inverted terminal repeats (ITRs) are key elements of AAV. These guanine-cytosine-rich structures are involved in the replication and encapsidation of the AAV genome, along with its integration in and excision from the… read more here.
Keywords: system; production; dna; itr ... See more keywords
Degradation and stable maintenance of adeno-associated virus inverted terminal repeats in E. coli
Sign Up to like & getrecommendations! Published in 2024 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkae1170
Abstract: Abstract Current plasmid propagation in E. coli compromises large inverted repeats, such as inverted terminal repeats (ITRs) of adeno-associated virus (AAV). Direct long-read sequencing analyses upon varying strains and culture conditions revealed ITR instability caused… read more here.
Keywords: adeno associated; degradation stable; inverted terminal; associated virus ... See more keywords