Preparation and Co‐Culture of iPSC‐Derived Dopaminergic Neurons and Astrocytes
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Cell Biology"
DOI: 10.1002/cpcb.98
Abstract: Induced pluripotent stem cell (iPSC)‐based models are powerful tools to study neurodegenerative diseases such as Parkinson's disease. The differentiation of patient‐derived neurons and astrocytes allows investigation of the molecular mechanisms responsible for disease onset and… read more here.
Keywords: dopaminergic neurons; preparation culture; ipsc; neurons astrocytes ... See more keywords
Recent progress of iPSC technology in cardiac diseases
Sign Up to like & getrecommendations! Published in 2021 at "Archives of Toxicology"
DOI: 10.1007/s00204-021-03172-3
Abstract: It has been nearly 15 years since the discovery of human-induced pluripotent stem cells (iPSCs). During this time, differentiation methods to targeted cells have dramatically improved, and many types of cells in the human body… read more here.
Keywords: technology cardiac; ipsc technology; ipsc; recent progress ... See more keywords
Mental health dished up—the use of iPSC models in neuropsychiatric research
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Neural Transmission"
DOI: 10.1007/s00702-020-02197-9
Abstract: Genetic and molecular mechanisms that play a causal role in mental illnesses are challenging to elucidate, particularly as there is a lack of relevant in vitro and in vivo models. However, the advent of induced… read more here.
Keywords: use; psychiatric disease; disorder; ipsc ... See more keywords
Deconvoluting the Cells of the Human Heart with iPSC Technology: Cell Types, Protocols, and Uses
Sign Up to like & getrecommendations! Published in 2022 at "Current Cardiology Reports"
DOI: 10.1007/s11886-022-01670-z
Abstract: Induced pluripotent stem cells (iPSCs) have become widely adopted tools in cardiovascular biology due to their ability to differentiate into patient-specific cell types. Here, we describe the current protocols, important discoveries, and experimental limitations from… read more here.
Keywords: human heart; cell; ipsc; deconvoluting cells ... See more keywords
Murine iPSC-derived microglia and macrophage cell culture models recapitulate distinct phenotypical and functional properties of classical and alternative neuro-immune polarisation
Sign Up to like & getrecommendations! Published in 2019 at "Brain, Behavior, and Immunity"
DOI: 10.1016/j.bbi.2019.09.009
Abstract: The establishment and validation of reliable induced pluripotent stem cell (iPSC)-derived in vitro models to study microglia and monocyte/macrophage immune function holds great potential for fundamental and translational neuro-immunology research. In this study, we first… read more here.
Keywords: immune polarisation; murine ipsc; ipsc; ipsc microglia ... See more keywords
Back and forth in time: Directing age in iPSC-derived lineages
Sign Up to like & getrecommendations! Published in 2017 at "Brain Research"
DOI: 10.1016/j.brainres.2015.11.013
Abstract: The advent of induced pluripotent stem cells (iPSC) has transformed the classic approach of studying human disease, providing in vitro access to disease-relevant cells from patients for the study of disease pathogenesis and for drug… read more here.
Keywords: time directing; age; back forth; ipsc ... See more keywords
Modeling iPSC-derived human neurofibroma-like tumors in mice uncovers the heterogeneity of Schwann cells within plexiform neurofibromas.
Sign Up to like & getrecommendations! Published in 2022 at "Cell reports"
DOI: 10.1016/j.celrep.2022.110385
Abstract: Plexiform neurofibromas (pNFs) are developmental tumors that appear in neurofibromatosis type 1 individuals, constituting a major source of morbidity and potentially transforming into a highly metastatic sarcoma (MPNST). pNFs arise after NF1 inactivation in a… read more here.
Keywords: like tumors; ipsc derived; ipsc; modeling ipsc ... See more keywords
Cross-Comparison of Human iPSC Motor Neuron Models of Familial and Sporadic ALS Reveals Early and Convergent Transcriptomic Disease Signatures.
Sign Up to like & getrecommendations! Published in 2020 at "Cell systems"
DOI: 10.1016/j.cels.2020.10.010
Abstract: Induced pluripotent stem cell (iPSC)-derived neural cultures from amyotrophic lateral sclerosis (ALS) patients can model disease phenotypes. However, heterogeneity arising from genetic and experimental variability limits their utility, impacting reproducibility and the ability to track… read more here.
Keywords: early convergent; disease; cross comparison; sporadic als ... See more keywords
Establishing iPSC cell banks derived using reagents and workflows optimized for cell therapy manufacturing
Sign Up to like & getrecommendations! Published in 2019 at "Cytotherapy"
DOI: 10.1016/j.jcyt.2019.03.574
Abstract: Background & Aim Induced pluripotent stem cell (iPSC) research is rapidly moving towards translational and clinical applications.These applications require robust and consistent workflows that utilize high quality reagents that are xeno-free.When commonly used animal-origin components… read more here.
Keywords: quality; establishing ipsc; xeno free; donor ... See more keywords
Effective regeneration of dystrophic muscle using autologous iPSC-derived progenitors with CRISPR-Cas9 mediated precise correction.
Sign Up to like & getrecommendations! Published in 2018 at "Medical hypotheses"
DOI: 10.1016/j.mehy.2017.11.009
Abstract: Duchenne muscular dystrophy (DMD) is a lethal muscle wasting disease caused by a lack of dystrophin, which eventually leads to apoptosis of muscle cells and impaired muscle contractility. Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated… read more here.
Keywords: muscle; crispr cas9; effective regeneration; ipsc ... See more keywords
Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts.
Sign Up to like & getrecommendations! Published in 2017 at "Stem cell research"
DOI: 10.1016/j.scr.2016.12.004
Abstract: Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced… read more here.
Keywords: mml 6838; 6838 cl2; ipsc line; ipsc ... See more keywords