Human iPSC‐derived microglia: A growing toolset to study the brain's innate immune cells
Sign Up to like & getrecommendations! Published in 2020 at "Glia"
DOI: 10.1002/glia.23781
Abstract: Recent advances in the generation of microglia from human induced pluripotent stem cells (iPSCs) have provided exciting new approaches to examine and decipher the biology of microglia. As these techniques continue to evolve to encompass… read more here.
Keywords: microglia growing; toolset; biology; derived microglia ... See more keywords
Microglia integration into human midbrain organoids leads to increased neuronal maturation and functionality
Sign Up to like & getrecommendations! Published in 2022 at "Glia"
DOI: 10.1002/glia.24167
Abstract: The human brain is a complex, three-dimensional structure. To better recapitulate brain complexity, recent efforts have focused on the development of human specific midbrain organoids. Human iPSC-derived midbrain organoids consist of differentiated and functional neurons,… read more here.
Keywords: ipsc derived; integration human; microglia midbrain; microglia integration ... See more keywords
Human iPSC-Derived Proinflammatory Macrophages cause Insulin Resistance in an Isogenic White Adipose Tissue Microphysiological System.
Sign Up to like & getrecommendations! Published in 2023 at "Small"
DOI: 10.1002/smll.202203725
Abstract: Chronic white adipose tissue (WAT) inflammation has been recognized as a critical early event in the pathogenesis of obesity-related disorders. This process is characterized by the increased residency of proinflammatory M1 macrophages in WAT. However,… read more here.
Keywords: microphysiological system; ipsc derived; adipose tissue; white adipose ... See more keywords
Barrier Properties and Transcriptome Expression in Human iPSC‐Derived Models of the Blood–Brain Barrier
Sign Up to like & getrecommendations! Published in 2018 at "STEM CELLS"
DOI: 10.1002/stem.2908
Abstract: Cell‐based models of the blood–brain barrier (BBB) are important for increasing the knowledge of BBB formation, degradation and brain exposure of drug substances. Human models are preferred over animal models because of interspecies differences in… read more here.
Keywords: barrier; bbb; coculture; ipsc derived ... See more keywords
High-Content Analysis of Mitochondrial Function in iPSC-Derived Neurons.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9477-9_16
Abstract: Mitochondrial dysfunction is linked to many neurological diseases; therefore, the ability to measure mitochondrial function is of great use for researching disease and testing potential therapeutics. Here we describe a high-content assay to simultaneously measure… read more here.
Keywords: mitochondrial function; analysis; derived neurons; ipsc derived ... See more keywords
Modeling Psychiatric Diseases with Induced Pluripotent Stem Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-981-32-9721-0_15
Abstract: Neuropsychiatric disorders are a heterogeneous group of disorders that are challenging to model and treat, due to their underlying complex genetic architecture and clinical variability. Presently, increasingly more studies are making use of induced pluripotent… read more here.
Keywords: derived neurons; biology; pluripotent stem; induced pluripotent ... See more keywords
Insights into the identification of a molecular signature for amyotrophic lateral sclerosis exploiting integrated microRNA profiling of iPSC-derived motor neurons and exosomes
Sign Up to like & getrecommendations! Published in 2022 at "Cellular and Molecular Life Sciences"
DOI: 10.1007/s00018-022-04217-1
Abstract: Amyotrophic lateral sclerosis (ALS) is a rare neurodegenerative disorder characterized by progressive degeneration of motor neurons (MNs). Most cases are sporadic, whereas 10% are familial. The pathological mechanisms underlying the disease are partially understood, but… read more here.
Keywords: lateral sclerosis; ipsc derived; amyotrophic lateral; motor neurons ... See more keywords
Apolipoprotein E regulates lipid metabolism and α-synuclein pathology in human iPSC-derived cerebral organoids
Sign Up to like & getrecommendations! Published in 2021 at "Acta Neuropathologica"
DOI: 10.1007/s00401-021-02361-9
Abstract: APOE4 is a strong genetic risk factor for Alzheimer’s disease and Dementia with Lewy bodies; however, how its expression impacts pathogenic pathways in a human-relevant system is not clear. Here using human iPSC-derived cerebral organoid… read more here.
Keywords: pathology; cerebral organoids; derived cerebral; ipsc derived ... See more keywords
Down Syndrome iPSC-Derived Astrocytes Impair Neuronal Synaptogenesis and the mTOR Pathway In Vitro
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Neurobiology"
DOI: 10.1007/s12035-017-0818-6
Abstract: Several methods have been used to study the neuropathogenesis of Down syndrome (DS), such as mouse aneuploidies, post mortem human brains, and in vitro cell culture of neural progenitor cells. More recently, induced pluripotent stem… read more here.
Keywords: astrocytes impair; syndrome ipsc; mtor; ipsc derived ... See more keywords
Transcriptome Analysis of iPSC-Derived Neurons from Rubinstein-Taybi Patients Reveals Deficits in Neuronal Differentiation
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Neurobiology"
DOI: 10.1007/s12035-020-01983-6
Abstract: Rubinstein-Taybi syndrome (RSTS) is a rare multisystem developmental disorder with moderate to severe intellectual disability caused by heterozygous mutations of either CREBBP or EP300 genes encoding CBP/p300 chromatin regulators. We explored the gene programs and… read more here.
Keywords: transcriptome analysis; derived neurons; rubinstein taybi; differentiation ... See more keywords
IPSC-Derived Neurons Harboring a Known Epilepsy Mutation Display Known and Novel Electrophysiological Phenotypes
Sign Up to like & getrecommendations! Published in 2017 at "Biophysical Journal"
DOI: 10.1016/j.bpj.2016.11.2954
Abstract: Epilepsy is a disturbance in the electrical activity of the brain manifested via countless etiologies. 65 million individuals suffer from epilepsy and one-third of these individuals live with uncontrollable seizures because no known pharmacological treatment… read more here.
Keywords: medicine; neurons harboring; single gene; ipsc derived ... See more keywords