Establishment of a low‐dosage‐IPTG inducible expression system construction method in Escherichia coli
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201800160
Abstract: The lac operon is a delicate inducible gene expression element in bacteria. To efficiently induce gene expression, a sufficient dosage of an inducer, usually that of 500–1000 µM isopropyl β‐D‐1‐thiogalactopyranoside (IPTG), is required to keep repressor… read more here.
Keywords: expression; iptg inducible; dosage iptg; inducible expression ... See more keywords
Multiplexed characterization of rationally designed promoter architectures deconstructs combinatorial logic for IPTG-inducible systems
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-020-20094-3
Abstract: A crucial step towards engineering biological systems is the ability to precisely tune the genetic response to environmental stimuli. In the case of Escherichia coli inducible promoters, our incomplete understanding of the relationship between sequence… read more here.
Keywords: inducible promoters; expression; iptg inducible; promoter ... See more keywords