Isobaric labeling: Expanding the breadth, accuracy, depth, and diversity of sample multiplexing
Sign Up to like & getrecommendations! Published in 2022 at "PROTEOMICS"
DOI: 10.1002/pmic.202200328
Abstract: Isobaric labeling has rapidly become a predominant strategy for proteome‐wide abundance measurements. Coupled to mass spectrometry, sample multiplexing techniques using isobaric labeling are unparalleled for profiling proteins and posttranslational modifications across multiple samples in a… read more here.
Keywords: sample multiplexing; depth; accuracy; expanding breadth ... See more keywords
Isobaric Labeling-Based LC-MS/MS Strategy for Comprehensive Profiling of Human Pancreatic Tissue Proteome.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/7651_2017_77
Abstract: The pancreas is an organ with both endocrine and exocrine functions, and various pathologies, such as pancreatic cancer and diabetes are associated with this organ. Owing to the limited pancreatic biopsy samples available for research,… read more here.
Keywords: tissue proteome; pancreatic tissue; tissue; human pancreatic ... See more keywords
New Set of Isobaric Labeling Reagents for Quantitative 16Plex Proteomics.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.3c00235
Abstract: Peptide labeling by isobaric tags is a powerful approach for the relative quantitative analysis of proteomes in multiple groups. There has been a revolution in the innovation of new isobaric reagents; however, great effort is… read more here.
Keywords: set isobaric; labeling reagents; ibt 16plex; reagents quantitative ... See more keywords
Reagents for Isobaric Labeling Peptides in Quantitative Proteomics.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b00321
Abstract: Currently, the commercial reagents for isobaric peptides labeling (TMT and iTRAQ) have some drawbacks, such as high cost in experiments, especially in quantitation for the modified peptides, and inconvenient handling for variable sizes of samples.… read more here.
Keywords: reagents isobaric; chemistry; labeling peptides; quantitative proteomics ... See more keywords
Boosting to Amplify Signal with Isobaric Labeling (BASIL) Strategy for Comprehensive Quantitative Phosphoproteomic Characterization of Small Populations of Cells.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b00024
Abstract: Comprehensive phosphoproteomic analysis of small populations of cells remains a daunting task due primarily to the insufficient MS signal intensity from low concentrations of enriched phosphopeptides. Isobaric labeling has a unique multiplexing feature where the… read more here.
Keywords: comprehensive quantitative; populations cells; small populations; quantitative phosphoproteomic ... See more keywords
Evaluation of a Dual Isolation Width Acquisition Method for Isobaric Labeling Ratio Decompression
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Proteome Research"
DOI: 10.1021/acs.jproteome.8b00870
Abstract: Isobaric labeling is a highly precise approach for protein quantification. However, due to the isolation interference problem, isobaric tagging suffers from ratio underestimation at the MS2 level. The use of narrow isolation widths is a… read more here.
Keywords: dual isolation; width acquisition; isobaric labeling; isolation width ... See more keywords
Multi-Q 2 software facilitates isobaric labeling quantitation analysis with improved accuracy and coverage
Sign Up to like & getrecommendations! Published in 2021 at "Scientific Reports"
DOI: 10.1038/s41598-021-81740-4
Abstract: Mass spectrometry-based proteomics using isobaric labeling for multiplex quantitation has become a popular approach for proteomic studies. We present Multi-Q 2, an isobaric-labeling quantitation tool which can yield the largest quantitation coverage and improved quantitation… read more here.
Keywords: labeling quantitation; multi; quantitation; analysis ... See more keywords