Design of experiments as a valuable tool for biopharmaceutical analysis with (imaged) capillary isoelectric focusing
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DOI: 10.1002/elps.201900162
Abstract: Capillary isoelectric focusing is indispensable for characterizing charge heterogeneity and isoelectric points of biopharmaceuticals. However, there are many influencing parameters and therefore method development is challenging. This study was performed to obtain an in‐depth understanding… read more here.
Keywords: capillary isoelectric; experiments valuable; valuable tool; design experiments ... See more keywords
Analysis of four therapeutic monoclonal antibodies by online capillary isoelectric focusing directly coupled to quadrupole time‐of‐flight mass spectrometry
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DOI: 10.1002/elps.201900195
Abstract: Capillary isoelectric focusing (cIEF) was online coupled to a Q‐TOF MS by a flow‐through microvial interface for the analysis of therapeutic mAb. Intact molecular weights obtained from the mass spectrum deconvolution of separated charge variants… read more here.
Keywords: capillary isoelectric; analysis four; analysis; mass ... See more keywords
Evolution of the theoretical description of the isoelectric focusing experiment: I. The path from Svensson's steady‐state model to the current two‐stage model of isoelectric focusing
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DOI: 10.1002/elps.202200236
Abstract: In 1961, Svensson described isoelectric focusing (IEF), the separation of ampholytic compounds in a stationary, natural pH gradient that was formed by passing current through a sucrose density gradient‐stabilized ampholyte mixture in a constant cross‐section… read more here.
Keywords: isoelectric focusing; two stage; gradient; svensson ... See more keywords
Evolution of the theoretical description of the isoelectric focusing experiment: II. An open system isoelectric focusing experiment is a transient, bidirectional isotachophoretic experiment
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DOI: 10.1002/elps.202200238
Abstract: The carrier ampholytes‐based (CA‐based) isoelectric focusing (IEF) experiment evolved from Svensson's closed system IEF (constant spatial current density, absence of convective mixing, counter‐balancing electrophoretic and diffusive fluxes yielding a steady state pH gradient) to the… read more here.
Keywords: isoelectric focusing; system ief; experiment; focusing experiment ... See more keywords
Electrophoretic mobilization in capillary isoelectric focusing by a weak acid or an acidic ampholyte as catholyte assessed by computer simulation
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DOI: 10.1002/elps.202200262
Abstract: Capillary isoelectric focusing (CIEF) with cationic electrophoretic mobilization induced via replacing the catholyte with the anolyte or a solution of another acid or amino acid was investigated by computer simulation for a wide range pH… read more here.
Keywords: isoelectric focusing; computer simulation; electrophoretic mobilization; acid ... See more keywords
Micro free‐flow isoelectric focusing with integrated optical pH sensors
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DOI: 10.1002/elsc.201700035
Abstract: Recently, a new observation method for monitoring of pH gradients in microfluidic free‐flow electrophoresis has emerged. It is based on the use of chip‐integrated fluorescent or luminescent micro sensor layers. These are able to monitor… read more here.
Keywords: focusing integrated; free flow; micro free; flow isoelectric ... See more keywords
Comparison between capillary zone electrophoresis and capillary isoelectric focusing for thalassemia screening in southern China
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DOI: 10.1002/jcla.22567
Abstract: Capillary isoelectric focusing is a type of capillary electrophoresis method newly used for thalassemia screening in China. Although the good performance has been proved by several studies, whether it can best suit the special needs… read more here.
Keywords: capillary isoelectric; comparison capillary; electrophoresis; thalassemia screening ... See more keywords
Preparative isoelectric focusing in a cellulose-based separation medium.
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DOI: 10.1002/jssc.201700036
Abstract: An improved preparative method based on isoelectric focusing of analytes in a cellulose-based separation medium is described in this study. Cellulose is suspended in an aqueous solution of simple buffers, ethylene glycol, glycerol, nonionic surfactant,… read more here.
Keywords: based separation; separation medium; separation; cellulose based ... See more keywords
Isoelectric Focusing on Non-Denaturing Flatbed Gels.
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DOI: 10.1007/978-1-4939-8793-1_9
Abstract: Isoelectric focusing (IEF) serves as a very useful procedure for cell protein separation and characterization. We have used this method to study antibody clonotype changes. Here we discuss the use of a sensitive native flatbed… read more here.
Keywords: focusing non; non denaturing; denaturing flatbed; flatbed gels ... See more keywords
Use of liquid isoelectric focusing (OFFGEL) on the discovery of meat tenderness biomarkers.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2018.05.005
Abstract: Protein biomarkers of meat tenderness are known to be of primary importance for the prediction of meat quality, and hence, industry profitability. Proteome analysis was performed on meat from 8 Main Anjou beef cattle, previously… read more here.
Keywords: protein; meat; liquid isoelectric; meat tenderness ... See more keywords
Search for proteomic biomarkers related to bovine pre-slaughter stress using liquid isoelectric focusing (OFFGEL) and mass spectrometry.
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DOI: 10.1016/j.jprot.2018.10.013
Abstract: Proteome changes derived from animals that have suffered pre-slaughter stress are a fact. In this study, Proteomic analysis was carried out on 20 bovine loin samples from Asturiana de los Valles and crossbreds cattle previously… read more here.
Keywords: pre slaughter; slaughter stress; liquid isoelectric; isoelectric focusing ... See more keywords