Impact of the isoelectric point of model parvoviruses on viral retention in anion‐exchange chromatography
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27555
Abstract: Anion‐exchange chromatography (AEX) is used in the downstream purification of monoclonal antibodies to remove impurities and potential viral contamination based on electrostatic interactions. Although the isoelectric point (pI) of viruses is considered a key factor… read more here.
Keywords: isoelectric point; anion exchange; exchange chromatography;
A simple method to estimate the isoelectric point of modified Tomato bushy stunt virus (TBSV) particles
Sign Up to like & getrecommendations! Published in 2017 at "ELECTROPHORESIS"
DOI: 10.1002/elps.201700209
Abstract: We present a simple method to estimate the isoelectric point (pI) of Tomato Bushy Stunt particles. We demonstrate that the combination of agarose gels with different pH buffers can be used to electrophorese the virus… read more here.
Keywords: isoelectric point; method estimate; estimate isoelectric; tomato bushy ... See more keywords
l-arginine directed and ultrasonically aided growth of nanocrystalline hydroxyapatite particles with tunable morphology
Sign Up to like & getrecommendations! Published in 2018 at "Colloids and Surfaces A: Physicochemical and Engineering Aspects"
DOI: 10.1016/j.colsurfa.2017.11.012
Abstract: Abstract Hydroxyapatite, Ca10(PO4)6(OH)2, a main inorganic component of bone material is widely used in various biomedical applications due to its excellent bioactivity and biocompatibility. Synthesizing hydroxyapatite of desired morphology by a prior and conscious manipulation… read more here.
Keywords: growth; isoelectric point; arginine; morphology ... See more keywords
Pore structure development of silica particles below the isoelectric point
Sign Up to like & getrecommendations! Published in 2018 at "Microporous and Mesoporous Materials"
DOI: 10.1016/j.micromeso.2018.03.031
Abstract: Abstract The textural properties of amorphous silica play a fundamental role in its final properties and, therefore, in its possible applications. The aim of this study is to gain comprehensive understanding about how the process… read more here.
Keywords: silica particles; silica; isoelectric point; pore structure ... See more keywords
Nanopore Current Enhancements Lack Protein Charge Dependence and Elucidate Maximum Unfolding at Protein's Isoelectric Point.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.1c11540
Abstract: Protein sequencing, as well as protein fingerprinting, has gained tremendous attention in the electrical sensing realm of solid-state nanopores and is challenging due to fast translocations and the use of high molar electrolytes. Despite providing… read more here.
Keywords: isoelectric point; charge; current enhancements; nanopore current ... See more keywords
Nanogel receptors for high isoelectric point protein detection: influence of electrostatic and covalent polymer-protein interactions.
Sign Up to like & getrecommendations! Published in 2020 at "Chemical communications"
DOI: 10.1039/d0cc02200d
Abstract: An aldehyde acrylate-based functional monomer was incorporated into poly(N-isopropylacrylamide-co-methacrylic acid) nanogels for use as protein receptors. The aldehyde component forms dynamic imines with surface exposed lysine residues, while carboxylic acid/carboxylate moieties form electrostatic interactions with… read more here.
Keywords: protein; isoelectric point; receptors high; high isoelectric ... See more keywords
Effect of ions on adsorption of lysozyme protein below its isoelectric point on hydrophilic (OH-Si) and hydrophobic (H-Si) surfaces
Sign Up to like & getrecommendations! Published in 2023 at "New Journal of Chemistry"
DOI: 10.1039/d3nj00624g
Abstract: Lysozyme is a rigid globular protein with four disulfide bonds which is present in human tears, saliva, sweat and milk. In the present study, thin films of this protein are... read more here.
Keywords: effect ions; isoelectric point; adsorption lysozyme; protein isoelectric ... See more keywords
Proteome-pI 2.0: proteome isoelectric point database update.
Sign Up to like & getrecommendations! Published in 2021 at "Nucleic acids research"
DOI: 10.1093/nar/gkab944
Abstract: Proteome-pI 2.0 is an update of an online database containing predicted isoelectric points and pKa dissociation constants of proteins and peptides. The isoelectric point-the pH at which a particular molecule carries no net electrical charge-is… read more here.
Keywords: isoelectric point; proteome isoelectric; proteome proteome; point ... See more keywords
Proteome-pI: proteome isoelectric point database
Sign Up to like & getrecommendations! Published in 2017 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkw978
Abstract: Abstract Proteome-pI is an online database containing information about predicted isoelectric points for 5029 proteomes calculated using 18 methods. The isoelectric point, the pH at which a particular molecule carries no net electrical charge, is… read more here.
Keywords: isoelectric point; proteome isoelectric; proteome proteome; point database ... See more keywords
Properties and Specific Functional Features of Wheat Grain α-Amylase/Subtilisin Inhibitor
Sign Up to like & getrecommendations! Published in 2018 at "Applied Biochemistry and Microbiology"
DOI: 10.1134/s0003683818020059
Abstract: A protein bifunctional inhibitor of endogenous α-amylase and subtilisin has been isolated from wheat grain and purified. The inhibitor specifically inactivates α-amylase isozymes with high isoelectric point values (group α-AMY1) and has almost no effect… read more here.
Keywords: wheat grain; inhibitor; isoelectric point; amylase subtilisin ... See more keywords