Single-cell Iso-Sequencing enables rapid genome annotation for scRNAseq analysis
Sign Up to like & getrecommendations! Published in 2022 at "Genetics"
DOI: 10.1093/genetics/iyac017
Abstract: Abstract Single-cell RNA sequencing is a powerful technique that continues to expand across various biological applications. However, incomplete 3′-UTR annotations can impede single-cell analysis resulting in genes that are partially or completely uncounted. Performing single-cell… read more here.
Keywords: rna sequencing; single cell; cell; cell isoform ... See more keywords
Microfluidic isoform sequencing shows widespread splicing coordination in the human transcriptome.
Sign Up to like & getrecommendations! Published in 2018 at "Genome research"
DOI: 10.1101/gr.230516.117
Abstract: Understanding transcriptome complexity is crucial for understanding human biology and disease. Technologies such as Synthetic long-read RNA sequencing (SLR-RNA-seq) delivered 5 million isoforms and allowed assessing splicing coordination. Pacific Biosciences and Oxford Nanopore increase throughput… read more here.
Keywords: isoform sequencing; coordination; human transcriptome; splicing coordination ... See more keywords