Isolation and Culture of Microglia
Sign Up to like & getrecommendations! Published in 2019 at "Current Protocols in Immunology"
DOI: 10.1002/cpim.70
Abstract: Microglia represent 5‐10% of cells in the central nervous system and contribute to the development, homeostasis, injury, and repair of neural tissues. As the tissue‐resident macrophages of the central nervous system, microglia execute core innate… read more here.
Keywords: culture microglia; immunology; isolation culture;
Isolation, Culture, and Motility Measurements of Epidermal Melanocytes from GFP-Expressing Reporter Mice.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/7651_2018_116
Abstract: In this article, we provide a method to isolate primary epidermal melanocytes from reporter mice, which also allow targeted gene inactivation. The mice from which these cells are isolated are bred into a Rosa26mT/mG reporter… read more here.
Keywords: reporter; reporter mice; isolation culture; epidermal melanocytes ... See more keywords
Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/7651_2019_209
Abstract: The isolation and culture of single floating myofibers with their adjacent muscle stem cells allow the analysis and comparison of muscle stem cells from aged and young mice. This method has the advantage that muscle… read more here.
Keywords: muscle; stem cells; muscle stem; isolation culture ... See more keywords
Isolation and Culture of Leptospira from Clinical and Environmental Samples.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0459-5_1
Abstract: Leptospires, the etiological agents of leptospirosis, are fastidious slow-growing organisms. Here we describe the isolation and routine maintenance of leptospires from clinical (blood, urine, or tissue) and environmental (water or soil) samples. Using combinations of… read more here.
Keywords: clinical environmental; isolation culture; culture leptospira; environmental samples ... See more keywords
Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples.
Sign Up to like & getrecommendations! Published in 2020 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-0603-2_1
Abstract: The isolation of arbuscular mycorrhizal fungi from different land use is the starting point for selecting and producing inoculants. There are different techniques to isolate and produce large-scale arbuscular mycorrhizal fungi-based inoculum, being soil, inert… read more here.
Keywords: isolation culture; mycorrhizal fungi; arbuscular mycorrhizal; mycorrhizal ... See more keywords
Isolation, Culture, and Cryopreservation of Adult Rodent Schwann Cells Derived from Immediately Dissociated Teased Fibers.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7649-2_4
Abstract: Adult Schwann cell (SC) cultures are usually derived from nerves subjected to a lengthy step of pre-degeneration to facilitate enzymatic digestion and recovery of viable cells. To overcome the need for pre-degeneration, we developed a… read more here.
Keywords: schwann; adult; isolation culture; culture cryopreservation ... See more keywords
Isolation and culture of human primary keratinocytes—a methods review
Sign Up to like & getrecommendations! Published in 2019 at "Experimental Dermatology"
DOI: 10.1111/exd.13860
Abstract: Keratinocyte culture is a necessary and widely used tool in a variety of experimental dermatological and biomedical studies. Literature search has revealed a variety of different protocols of human primary keratinocyte isolation and culture. Therefore,… read more here.
Keywords: primary keratinocytes; keratinocytes methods; isolation culture; culture human ... See more keywords
Isolation and culture of porcine primary fetal progenitors and neurons from the developing dorsal telencephalon
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Veterinary Science"
DOI: 10.4142/jvs.2019.20.e3
Abstract: The development of long-term surviving fetal cell cultures from primary cell tissue from the developing brain is important for facilitating studies investigating neural development and for modelling neural disorders and brain congenital defects. The field… read more here.
Keywords: progenitors neurons; isolation culture; dorsal telencephalon; culture porcine ... See more keywords