New Approaches for Absolute Quantification of Stable‐Isotope‐Labeled Peptide Standards for Targeted Proteomics Based on a UV Active Tag
Sign Up to like & getrecommendations! Published in 2020 at "PROTEOMICS"
DOI: 10.1002/pmic.202000007
Abstract: Targeted proteomics depends on the availability of stable isotope labeled (SIL) peptide standards, which for absolute protein quantification need to be absolutely quantified. In the present study, three new approaches for absolute quantification of SIL… read more here.
Keywords: peptide standards; new approaches; targeted proteomics; stable isotope ... See more keywords
A quantitation module for isotope-labeled peptides integrated into PatternLab for proteomics.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteomics"
DOI: 10.1016/j.jprot.2019.04.021
Abstract: We present a new module integrated into the widely adopted PatternLab for proteomics to enable analysis of isotope-labeled peptides produced using dimethyl or SILAC. The accurate quantitation of proteins lies within the heart of proteomics;… read more here.
Keywords: module; quantitation; patternlab proteomics; isotope labeled ... See more keywords
Streamlined Subclass-Specific Absolute Quantification of Serum IgG Glycopeptides Using Synthetic Isotope-Labeled Standards.
Sign Up to like & getrecommendations! Published in 2021 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.0c04462
Abstract: Absolute glycoproteomics quantification has drawn tremendous attention owing to its prospects in biomarker discovery and clinical implementation but is impeded by a general lack of suitable heavy isotope-labeled glycopeptide standards. In this study, we devised… read more here.
Keywords: absolute quantification; igg glycopeptides; isotope labeled;
Simultaneous Relative Quantification of Various Polyglycerophospholipids with Isotope-Labeled Methylation by Nanoflow Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b00800
Abstract: Herein, we introduce a comprehensive analytical method for the separation and relative quantification of polyglycerophospholipids (PGPLs), including phosphatidylglycerol (PG), bis(monoacylglycero)phosphate (BMP), bis(diacylglycero)phosphate (BDP), Hemi BDP, cardiolipin (CL), monolysocardiolipin (MLCL), and dilysocardiolipin (DLCL), using isotope-labeled methylation… read more here.
Keywords: labeled methylation; bdp; isotope labeled; relative quantification ... See more keywords
LC-MS/MS-PRM Quantification of IgG Glycoforms Using Stable Isotope Labeled IgG1 Fc Glycopeptide Standard.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.2c00475
Abstract: Targeted quantification of proteins is a standard methodology with broad utility, but targeted quantification of glycoproteins has not reached its full potential. The lack of optimized workflows and isotopically labeled standards limits the acceptance of… read more here.
Keywords: quantification igg; isotope labeled; stable isotope; quantification ... See more keywords
Mass Spectrometry-Based Quantification of Tau in Human Cerebrospinal Fluid Using a Complementary Tryptic Peptide Standard.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.8b00920
Abstract: Here, we report a method for the generation of complementary tryptic (CompTryp) isotope-labeled peptide standards for the relative and absolute quantification of proteins by mass spectrometry (MS). These standards can be digested in parallel with… read more here.
Keywords: human cerebrospinal; tau; mass spectrometry; isotope labeled ... See more keywords
The Isotope-Labeled, MS-Cleavable Cross-Linker Disuccinimidyl Dibutyric Urea for Improved Cross-Linking/Mass Spectrometry Studies.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.9b00008
Abstract: Previous studies have shown the benefits of the amine-reactive, CID-MS/MS-cleavable cross-linker disuccinimidyl dibutyric urea (DSBU) for structural proteomics studies via cross-linking/MS (XL-MS). To further facilitate the automation of XL-MS experiments, we synthesized a deuterated (D12)… read more here.
Keywords: mass; cross; cross linker; isotope labeled ... See more keywords
High-sensitivity tracing of stable isotope labeled Ag nanoparticles in environmental samples using MC-ICP-MS
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Analytical Atomic Spectrometry"
DOI: 10.1039/c8ja00358k
Abstract: Silver nanoparticles are among the most widely used engineered nanomaterials and this warrants further investigation of their behaviour and fate in the environment. read more here.
Keywords: isotope labeled; labeled nanoparticles; sensitivity tracing; stable isotope ... See more keywords
A Method to Evaluate Isotopic and Energy Turnover Rates in Larval Culex quinquefasciatus (Diptera: Culicidae) Using Stable Isotope Labeled Compounds
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Medical Entomology"
DOI: 10.1093/jme/tjy025
Abstract: Abstract The goal of this study was to evaluate the use of stable isotope labeled compounds to better understand factors influencing energy turnover in larval Culex quinquefasciatus (Say; Diptera: Culicidae). Three isotope labeled compounds were… read more here.
Keywords: labeled compounds; turnover; energy turnover; isotope labeled ... See more keywords
Isotope‐labeled amyloid‐β does not transmit to the brain in a prion‐like manner after peripheral administration
Sign Up to like & getrecommendations! Published in 2022 at "EMBO Reports"
DOI: 10.15252/embr.202154405
Abstract: Findings of early cerebral amyloid‐β deposition in mice after peripheral injection of amyloid‐β‐containing brain extracts, and in humans following cadaveric human growth hormone treatment raised concerns that amyloid‐β aggregates and possibly Alzheimer’s disease may be… read more here.
Keywords: isotope labeled; administration; brain extracts; brain ... See more keywords