Analysis of the Global Changes in SH2 Binding Properties Using Mass Spectrometry Supported by Quantitative Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technique.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6762-9_24
Abstract: Quantitative mass spectrometry (MS)-based proteomics enables fast and reliable analysis of protein complexes. Its robustness and sensitivity effectively substitute traditional antibody-based approaches. Here, we describe the combination of mass spectrometry and Stable Isotope Labeling by… read more here.
Keywords: isotope labeling; labeling amino; mass spectrometry; mass ... See more keywords
Chemical Isotope Labeling LC-MS for Metabolomics.
Sign Up to like & getrecommendations! Published in 2021 at "Advances in experimental medicine and biology"
DOI: 10.1007/978-3-030-51652-9_1
Abstract: Due to the great diversity of chemical and physical properties of metabolites as well as a wide range of concentrations of metabolites present in metabolomic samples, performing comprehensive and quantitative metabolome analysis is a major… read more here.
Keywords: chemical isotope; coverage; labeling metabolomics; isotope labeling ... See more keywords
Isotope labeling for studying RNA by solid-state NMR spectroscopy
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Biomolecular NMR"
DOI: 10.1007/s10858-018-0180-7
Abstract: Nucleic acids play key roles in most biological processes, either in isolation or in complex with proteins. Often they are difficult targets for structural studies, due to their dynamic behavior and high molecular weight. Solid-state… read more here.
Keywords: solid state; isotope labeling; studying rna; spectroscopy ... See more keywords
Simultaneous quantification of free amino acids and 5'-nucleotides in shiitake mushrooms by stable isotope labeling-LC-MS/MS analysis.
Sign Up to like & getrecommendations! Published in 2018 at "Food chemistry"
DOI: 10.1016/j.foodchem.2018.06.054
Abstract: Evaluation of free amino acids (FAAs) and nucleotides in various food matrices has been a widely studied topic in recent years. Here, a fast and efficient strategy for the simultaneous analysis of 20 FAAs and… read more here.
Keywords: isotope labeling; amino acids; analysis; stable isotope ... See more keywords
Identification of naringin metabolites mediated by human intestinal microbes with stable isotope‐labeling method and UFLC‐Q‐TOF‐MS/MS
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Pharmaceutical and Biomedical Analysis"
DOI: 10.1016/j.jpba.2018.08.039
Abstract: HighlightsA total of 13 naringin metabolites mediated by human intestinal microbes were identified, among which 5 were reported for the first time.Naringin underwent extensive phase I metabolism in human intestinal microbes.Microbial metabolic profiles of naringin… read more here.
Keywords: intestinal microbes; isotope labeling; mediated human; labeling method ... See more keywords
Stable isotope labeling derivatization combined with multiple-mass spectrometry technologies to monitor metabolites of tenuifoliside A incubated with intestinal bacteria incubation model.
Sign Up to like & getrecommendations! Published in 2021 at "Talanta"
DOI: 10.1016/j.talanta.2020.121791
Abstract: Aromatic carboxylic acids (ACAs), play important roles in preventive and therapeutic effects for some diseases. However, complex matrix effect and poor detection sensitivity make it difficult and even rare to detect ACAs in complex bio-samples.… read more here.
Keywords: bacteria incubation; isotope labeling; intestinal bacteria; stable isotope ... See more keywords
Development of Chemical Isotope Labeling Liquid Chromatography Orbitrap Mass Spectrometry for Comprehensive Analysis of Dipeptides.
Sign Up to like & getrecommendations! Published in 2023 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c05796
Abstract: Dipeptides have recently attracted considerable attention due to their newly found biological functions and potential biomarkers of diseases. Global analysis of dipeptides (400 common dipeptides in total number) in samples of complex matrices would enable… read more here.
Keywords: orbitrap; isotope labeling; liquid chromatography; analysis dipeptides ... See more keywords
Overcoming Sample Matrix Effect in Quantitative Blood Metabolomics Using Chemical Isotope Labeling Liquid Chromatography Mass Spectrometry.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.7b02240
Abstract: Blood is widely used for discovery metabolomics to search for disease biomarkers. However, blood sample matrix can have a profound effect on metabolome analysis, which can impose an undesirable restriction on the type of blood… read more here.
Keywords: isotope labeling; sample matrix; effect; blood ... See more keywords
In Vivo Stable-Isotope Labeling and Mass-Spectrometry-Based Metabolic Profiling of a Potent Tobacco-Specific Carcinogen in Rats.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01881
Abstract: The tobacco-specific nitrosamine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), is a potent lung carcinogen that exerts its carcinogenic effects upon metabolic activation. The identification and quantitation of NNK metabolites could identify potential biomarkers of bioactivation and detoxification of this… read more here.
Keywords: isotope labeling; mass spectrometry; nnk metabolites; tobacco specific ... See more keywords
Isotope Labeling to Study the Hydrogen Transfer Route during Lignite Modification in a Subcritical D2O–CO System
Sign Up to like & getrecommendations! Published in 2020 at "Energy & Fuels"
DOI: 10.1021/acs.energyfuels.9b04472
Abstract: The hydrogen transfer reaction is one of the key chemical processes of coal conversion. The elucidation of the hydrogen transfer mechanism is essential for rational control of the properties and di... read more here.
Keywords: isotope labeling; hydrogen; transfer route; labeling study ... See more keywords
Permanently positively charged stable isotope labeling agents and its application in the accurate quantitation of alkylphenols migrated from plastics to edible oils.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.0c03413
Abstract: A new permanently positively charged stable isotope labeling (SIL) agent pair, 4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)-N,N,N-trimethylbenzenaminium iodide（DPTBA）and its deuterated counterpart d3-DPTBA, was designed and synthesized. The SIL agents were applied to the liquid chromatography-tandem mass spectrometry analysis of alkylphenols.… read more here.
Keywords: permanently positively; isotope labeling; charged stable; alkylphenols migrated ... See more keywords