Stable Isotope Probing of Microorganisms in Environmental Samples with H218O.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9721-3_10
Abstract: We describe a protocol for investigating microbial growth in environmental samples via stable isotope probing (SIP) with H218O. Water is a universal substrate for all microorganisms and replication is required for DNA to become labeled… read more here.
Keywords: isotope probing; microorganisms environmental; environmental samples; stable isotope ... See more keywords
Profiling of Active Microorganisms by Stable Isotope Probing-Metagenomics.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9721-3_12
Abstract: Stable isotope probing (SIP) provides researchers a culture-independent method to retrieve nucleic acids from active microbial populations performing a specific metabolic activity in complex ecosystems. In recent years, the use of the SIP method in… read more here.
Keywords: isotope probing; active microorganisms; stable isotope; ecology ... See more keywords
Stable Isotope Probing of Microbial Phospholipid Fatty Acids in Environmental Samples.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9721-3_4
Abstract: Phospholipid fatty acid (PLFA) extracted from environmental samples describe the microbial community pattern and are sensitive to monitor and quantify shifts in the microbial community. Linkage with the stable isotope technique adds a functional perspective… read more here.
Keywords: isotope probing; isotope; probing microbial; environmental samples ... See more keywords
Data Analysis for DNA Stable Isotope Probing Experiments Using Multiple Window High-Resolution SIP.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9721-3_9
Abstract: DNA stable isotope probing (DNA-SIP) allows for the identification of microbes that assimilate isotopically labeled substrates into DNA. Here we describe the analysis of sequencing data using the multiple window high-resolution DNA-SIP method (MW-HR-SIP). MW-HR-SIP… read more here.
Keywords: dna stable; isotope probing; dna; analysis ... See more keywords
Unraveling uncultivable pesticide degraders via stable isotope probing (SIP)
Sign Up to like & getrecommendations! Published in 2018 at "Critical Reviews in Biotechnology"
DOI: 10.1080/07388551.2018.1427697
Abstract: Abstract Uncultivable microorganisms account for over 99% of all species on earth, playing essential roles in ecological processes such as carbon/nitrogen cycle and chemical mineralization. Their functions remain unclear in ecosystems and natural habitats, requiring… read more here.
Keywords: pesticide degraders; isotope probing; probing sip; pesticide ... See more keywords
Identification of active denitrifiers by DNA‐stable isotope probing and amplicon sequencing reveals Betaproteobacteria as responsible for attenuation of nitrate contamination in a low impacted aquifer
Sign Up to like & getrecommendations! Published in 2018 at "FEMS Microbiology Ecology"
DOI: 10.1093/femsec/fix181
Abstract: ABSTRACT Groundwater reservoirs constitute important freshwater resources. However, these ecosystems are highly vulnerable to contamination and have to rely on the resident microbiota to attenuate the impact of this contamination. Nitrate is one of the… read more here.
Keywords: dna stable; isotope probing; impacted aquifer; low impacted ... See more keywords
Anaerobic sulfamethoxazole-degrading bacterial consortia in antibiotic-contaminated wetland sediments identified by DNA-stable isotope probing and metagenomics analysis.
Sign Up to like & getrecommendations! Published in 2022 at "Environmental microbiology"
DOI: 10.1111/1462-2920.16091
Abstract: Anaerobic degradation has been demonstrated as an important pathway for the removal of sulfonamide (SA) in contaminated environments, and identifying the microorganisms responsible for the degradation of SA is a key step in developing bioaugmentation… read more here.
Keywords: stable isotope; dna; smx; wetland sediments ... See more keywords
Genomic Features Predict Bacterial Life History Strategies in Soil, as Identified by Metagenomic Stable Isotope Probing
Sign Up to like & getrecommendations! Published in 2022 at "mBio"
DOI: 10.1128/mbio.03584-22
Abstract: Soil microbes are major players in the global carbon cycle, yet we still have little understanding of how the carbon cycle operates in soil communities. A major limitation is that carbon metabolism lacks discrete functional… read more here.
Keywords: stable isotope; carbon; isotope probing; life history ... See more keywords
Measurement Error and Resolution in Quantitative Stable Isotope Probing: Implications for Experimental Design
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DOI: 10.1128/msystems.00151-20
Abstract: One of the biggest challenges in microbial ecology is correlating the identity of microorganisms with the roles they fulfill in natural environmental systems. Studies of microbes in pure culture reveal much about their genomic content… read more here.
Keywords: community; isotope; quantitative stable; experimental design ... See more keywords
New insight into the mechanisms of autochthonous fungal bioaugmentation of phenanthrene in petroleum contaminated soil by stable isotope probing.
Sign Up to like & getrecommendations! Published in 2023 at "Journal of hazardous materials"
DOI: 10.2139/ssrn.4326391
Abstract: Autochthonous fungal bioaugmentation (AFB) is considered a reliable bioremediation approach for polycyclic aromatic hydrocarbon (PAH) contamination, but little is known about its mechanisms in contaminated soils. Here, a microcosm experiment was performed to explore the… read more here.
Keywords: autochthonous fungal; bioaugmentation; stable isotope; phenanthrene ... See more keywords
Novel Isoprene-Degrading Proteobacteria From Soil and Leaves Identified by Cultivation and Metagenomics Analysis of Stable Isotope Probing Experiments
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.02700
Abstract: Isoprene is a climate-active gas and one of the most abundant biogenic volatile organic compounds (BVOC) released into the atmosphere. In the terrestrial environment, plants are the primary producers of isoprene, releasing between 500 and… read more here.
Keywords: isotope probing; analysis; isoprene degrading; novel isoprene ... See more keywords