Isotopically Labeled Clickable Glutathione to Quantify Protein S‐Glutathionylation
Sign Up to like & getrecommendations! Published in 2019 at "ChemBioChem"
DOI: 10.1002/cbic.201900528
Abstract: Protein S‐glutathionylation is one of the important cysteine oxidation events that regulate various redox‐mediated biological processes. Despite several existing methods, there are few proteomic approaches to identify and quantify specific cysteine residues susceptible to S‐glutathionylation.… read more here.
Keywords: glutathionylation; labeled clickable; protein glutathionylation; isotopically labeled ... See more keywords
HPLC–free method of synthesis of isotopically labeled deoxyfructosylated peptides
Sign Up to like & getrecommendations! Published in 2022 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-022-04022-6
Abstract: The biomarker strategy, based on multiple specific glycation sites in plasma proteins, could essentially increase the efficiency of glycemic control and disease prediction. Besides glycated albumin being a potential biomarker of early states of diabetes… read more here.
Keywords: labeled deoxyfructosylated; synthesis isotopically; glycation; method ... See more keywords
Isotopically-Labeled Iodoacetamide-Alkyne Probes for Quantitative Cysteine-Reactivity Profiling.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular pharmaceutics"
DOI: 10.1021/acs.molpharmaceut.7b00832
Abstract: Cysteine residues on proteins serve a variety of catalytic and regulatory functions due to the high nucleophilicity and redox activity of the thiol group. Quantitative proteomic platforms for profiling cysteine reactivity can provide valuable information… read more here.
Keywords: reactivity profiling; cysteine; iodoacetamide alkyne; isotopically labeled ... See more keywords
PyFragMS—A Web Tool for the Investigation of the Collision-Induced Fragmentation Pathways
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DOI: 10.1021/acsomega.1c07272
Abstract: Dissociation induced by the accumulation of internal energy via collisions of ions with neutral molecules is one of the most important fragmentation techniques in mass spectrometry (MS), and the identification of small singly charged molecules… read more here.
Keywords: tool investigation; fragmentation; isotopically labeled; web tool ... See more keywords
Recombinant Expression and Chemical Amidation of Isotopically Labeled Native Melittin
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DOI: 10.1021/jacs.2c12631
Abstract: Post-translational modifications are ubiquitous in the eukaryotic proteome. However, these modifications are rarely incorporated in NMR studies of eukaryotic proteins, which are typically produced through recombinant expression in E. coli. Melittin is the primary peptide… read more here.
Keywords: recombinant expression; expression; chemical amidation; native melittin ... See more keywords
Erratum: Use of isotopically labeled substrates reveals kinetic differences between human and bacterial serine palmitoyltransferase
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Lipid Research"
DOI: 10.1194/jlr.err119000228
Abstract: Isotope labels are frequently used tools to track metabolites through complex biochemical pathways and to discern the mechanisms of enzyme-catalysed reactions. Isotopically-labelled Lserine is often used to monitor the activity of the first enzyme in… read more here.
Keywords: isotopically labeled; use isotopically; serine palmitoyltransferase; serine ... See more keywords