Hierarchical Nanostructuring Array Enhances Mid-Hybridization for Accurate Herbal Identification via ITS2 DNA Barcode.
Sign Up to like & getrecommendations! Published in 2019 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.9b04687
Abstract: It remains a technical challenge to accurately identify close species of herbal medicines, especially from adulterants, because of their highly identical phenotypes and chemical compositions. Here, we report a direct, sequencing-free, high-curvature nanostructuring-based electrochemical herb… read more here.
Keywords: its2; array; mid hybridization; hybridization ... See more keywords
Intragenomic sequence variations in the second internal transcribed spacer (ITS2) ribosomal DNA of the malaria vector Anopheles stephensi
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DOI: 10.1371/journal.pone.0253173
Abstract: Second Internal Transcribed Spacer (ITS2) ribosomal DNA (rDNA) sequence is a widely used molecular marker for species-identification or -delimitation due to observed concerted evolution which is believed to homogenize rDNA copies in an interbreeding population.… read more here.
Keywords: its2; dna; rdna; anopheles stephensi ... See more keywords
Characterizing nrDNA ITS1, 5.8S and ITS2 secondary structures and their phylogenetic utility in the legume tribe Hedysareae with special reference to Hedysarum
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DOI: 10.1371/journal.pone.0283847
Abstract: This is the first study to systematically evaluate rRNA secondary structures of Hedysareae with an emphasis on Hedysarum. ITS2 and 5.8S regions of the genus shared a common secondary structure with a four-fingered central loop,… read more here.
Keywords: secondary structures; characterizing nrdna; hedysarum; its2 ... See more keywords
Structure-Based GC Investigation Sheds New Light on ITS2 Evolution in Corydalis Species
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DOI: 10.3390/ijms24097716
Abstract: Guanine and cytosine (GC) content is a fundamental component of genetic diversity and essential for phylogenetic analyses. However, the GC content of the ribosomal internal transcribed spacer 2 (ITS2) remains unknown, despite the fact that… read more here.
Keywords: structure; based investigation; its2; corydalis species ... See more keywords