Kinetic and Thermodynamic Characterization of Human 4-Oxo-l-proline Reductase Catalysis
Sign Up to like & getrecommendations! Published in 2025 at "Biochemistry"
DOI: 10.1021/acs.biochem.4c00721
Abstract: The enzyme 4-oxo-l-proline reductase (BDH2) has recently been identified in humans. BDH2, previously thought to be a cytosolic (R)-3-hydroxybutyrate dehydrogenase, actually catalyzes the NADH-dependent reduction of 4-oxo-l-proline to cis-4-hydroxy-l-proline, a compound with known anticancer activity.… read more here.
Keywords: chemistry; proline; oxo proline; proline reductase ... See more keywords
Biochemical and structural characterization of tomato polyphenol oxidases provide novel insights into their substrate specificity
Sign Up to like & getrecommendations! Published in 2019 at "Scientific Reports"
DOI: 10.1038/s41598-019-39687-0
Abstract: Polyphenol oxidases (PPOs) contain the structurally similar enzymes tyrosinases (TYRs) and catechol oxidases (COs). Two cDNAs encoding pro-PPOs from tomato (Solanum lycopersicum) were cloned and heterologously expressed in Escherichia coli. The two pro-PPOs (SlPPO1-2) differ… read more here.
Keywords: polyphenol oxidases; tomato; kcat; polyphenol ... See more keywords
IECata: interpretable bilinear attention network and evidential deep learning improve the catalytic efficiency prediction of enzymes
Sign Up to like & getrecommendations! Published in 2025 at "Briefings in Bioinformatics"
DOI: 10.1093/bib/bbaf283
Abstract: Abstract Enzyme catalytic efficiency (kcat/Km) is a key parameter for identifying high-activity enzymes. Recently, deep learning techniques have demonstrated the potential for fast and accurate kcat/Km prediction. However, three challenges remain: (i) the limited size… read more here.
Keywords: deep learning; catalytic efficiency; evidential deep; prediction ... See more keywords