Probing Ion Binding in the Selectivity Filter of the KcsA Potassium Channel.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b01092
Abstract: In potassium (K+) channels, permeation, selectivity, and gating at the selectivity filter are all governed by the thermodynamics and kinetics of the ion-protein interactions. Specific contacts between the carbonyl groups from the Thr-Val-Gly-Tyr-Gly signature filter… read more here.
Keywords: ion binding; binding sites; ion; kcsa ... See more keywords
Anionic Phospholipids Shift the Conformational Equilibrium of the Selectivity Filter in the KcsA Channel to the Conductive Conformation: Predicted Consequences on Inactivation
Sign Up to like & getrecommendations! Published in 2023 at "Biomedicines"
DOI: 10.3390/biomedicines11051376
Abstract: Here, we report an allosteric effect of an anionic phospholipid on a model K+ channel, KcsA. The anionic lipid in mixed detergent–lipid micelles specifically induces a change in the conformational equilibrium of the channel selectivity… read more here.
Keywords: kcsa; conformational equilibrium; anionic lipid; selectivity filter ... See more keywords
Modulation of Function, Structure and Clustering of K+ Channels by Lipids: Lessons Learnt from KcsA
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21072554
Abstract: KcsA, a prokaryote tetrameric potassium channel, was the first ion channel ever to be structurally solved at high resolution. This, along with the ease of its expression and purification, made KcsA an experimental system of… read more here.
Keywords: protein; lipid binding; channel; structure ... See more keywords
Probing the Structural Dynamics of the Activation Gate of KcsA Using Homo-FRET Measurements
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DOI: 10.3390/ijms222111954
Abstract: The allosteric coupling between activation and inactivation processes is a common feature observed in K+ channels. Particularly, in the prokaryotic KcsA channel the K+ conduction process is controlled by the inner gate, which is activated… read more here.
Keywords: homo fret; homo; gate; fret measurements ... See more keywords