Structural characterization and Kemp eliminase activity of the Mycobacterium smegmatis Ketosteroid Isomerase.
Sign Up to like & getrecommendations! Published in 2021 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2021.05.007
Abstract: The Kemp elimination reaction, involving the ring-opening of benzoxazole and its derivatives under the action of natural enzymes or chemical catalysts, has been of interest to researchers since its discovery. Because this reaction does not… read more here.
Keywords: structural characterization; ketosteroid isomerase; kemp elimination; mycobacterium smegmatis ... See more keywords
Efficient Base-Catalyzed Kemp Elimination in an Engineered Ancestral Enzyme
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms23168934
Abstract: The routine generation of enzymes with completely new active sites is one of the major unsolved problems in protein engineering. Advances in this field have been so far modest, perhaps due, at least in part,… read more here.
Keywords: efficient base; kemp elimination; engineering; elimination ... See more keywords