Genetically stable CRISPR-based kill switches for engineered microbes
Sign Up to like & getrecommendations! Published in 2022 at "Nature Communications"
DOI: 10.1038/s41467-022-28163-5
Abstract: Microbial biocontainment is an essential goal for engineering safe, next-generation living therapeutics. However, the genetic stability of biocontainment circuits, including kill switches, is a challenge that must be addressed. Kill switches are among the most… read more here.
Keywords: crispr based; kill switch; kill; based kill ... See more keywords
Engineered Mycobacterium tuberculosis triple-kill-switch strain provides controlled tuberculosis infection in animal models
Sign Up to like & getrecommendations! Published in 2025 at "Nature Microbiology"
DOI: 10.1038/s41564-024-01913-5
Abstract: Human challenge experiments could accelerate tuberculosis vaccine development. This requires a safe Mycobacterium tuberculosis (Mtb) strain that can both replicate in the host and be reliably cleared. Here we genetically engineered Mtb strains encoding up… read more here.
Keywords: tuberculosis; infection; mycobacterium tuberculosis; strain ... See more keywords