Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-6421-5_17
Abstract: Kinetochores are large multiprotein complexes that drive mitotic chromosome movements by mechanically coupling them to the growing and shortening tips of spindle microtubules. Kinetochores are also regulatory hubs, somehow sensing when they are erroneously attached… read more here.
Keywords: resolution; individual kinetochores; kinetochore; fluorescence ... See more keywords
Plasticity in centromere organization and kinetochore composition: Lessons from diversity.
Sign Up to like & getrecommendations! Published in 2022 at "Current opinion in cell biology"
DOI: 10.1016/j.ceb.2021.12.007
Abstract: Kinetochores are the macromolecular protein complexes that govern chromosome movement by binding spindle microtubules during mitosis and meiosis. Centromeres are the specific chromosomal regions that serve as the platform on which kinetochores assemble. Despite their… read more here.
Keywords: kinetochore; organization kinetochore; plasticity centromere; centromere organization ... See more keywords
Meiotic Kinetochores Fragment into Multiple Lobes upon Cohesin Loss in Aging Eggs
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DOI: 10.1016/j.cub.2019.09.006
Abstract: Summary Chromosome segregation errors during female meiosis are a leading cause of pregnancy loss and human infertility. The segregation of chromosomes is driven by interactions between spindle microtubules and kinetochores. Kinetochores in mammalian oocytes are… read more here.
Keywords: cohesin loss; kinetochore; age; fragmentation ... See more keywords
Microtubule Attachment and Centromeric Tension Shape the Protein Architecture of the Human Kinetochore
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DOI: 10.1016/j.cub.2020.09.038
Abstract: The nanoscale protein architecture of the kinetochore plays an integral role in specifying the mechanisms underlying its functions in chromosome segregation. However, defining this architecture in human cells remains challenging because of the large size… read more here.
Keywords: kinetochore; architecture human; microtubule attachment; protein architecture ... See more keywords
Complete microtubule–kinetochore occupancy favours the segregation of merotelic attachments
Sign Up to like & getrecommendations! Published in 2018 at "Nature Communications"
DOI: 10.1038/s41467-018-04427-x
Abstract: Kinetochores are multi-protein complexes that power chromosome movements by tracking microtubules plus-ends in the mitotic spindle. Human kinetochores bind up to 20 microtubules, even though single microtubules can generate sufficient force to move chromosomes. Here,… read more here.
Keywords: microtubule; kinetochore; microtubule occupancy; favours segregation ... See more keywords
Cell cycle control of kinetochore assembly
Sign Up to like & getrecommendations! Published in 2022 at "Nucleus"
DOI: 10.1080/19491034.2022.2115246
Abstract: ABSTRACT The kinetochore is a large proteinaceous structure assembled on the centromeres of chromosomes. The complex machinery links chromosomes to the mitotic spindle and is essential for accurate chromosome segregation during cell division. The kinetochore… read more here.
Keywords: kinetochore; cell cycle; control kinetochore; cycle control ... See more keywords
Permitted and restricted steps of human kinetochore assembly in mitotic cell extracts
Sign Up to like & getrecommendations! Published in 2021 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e20-07-0461
Abstract: Mitotic kinetochores assemble via the hierarchical recruitment of numerous cytosolic components to the centromere region of each chromosome. However, how these orderly and localized interactions are achieved without spurious macromolecular assemblies forming from soluble kinetochore… read more here.
Keywords: mitotic cell; microtubule binding; kinetochore; cell ... See more keywords
Divergent polo boxes in KKT2 bind KKT1 to initiate the kinetochore assembly cascade in Trypanosoma brucei
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Biology of the Cell"
DOI: 10.1091/mbc.e22-07-0269-t
Abstract: Chromosome segregation requires assembly of the macromolecular kinetochore complex onto centromeric DNA. While most eukaryotes have canonical kinetochore proteins that are widely conserved among eukaryotes, evolutionarily divergent kinetoplastids have a unique set of kinetochore proteins.… read more here.
Keywords: polo boxes; kinetochore; kinetochore proteins; divergent polo ... See more keywords
Distinct Aurora B pools at the inner centromere and kinetochore have different contributions to meiotic and mitotic chromosome segregation.
Sign Up to like & getrecommendations! Published in 2023 at "Molecular biology of the cell"
DOI: 10.1091/mbc.e23-01-0014
Abstract: Proper chromosome segregation depends on establishment of bioriented kinetochore-microtubule attachments, which often requires multiple rounds of release and reattachment. Aurora B and C kinases phosphorylate kinetochore proteins to release tensionless attachments. Multiple pathways recruit Aurora… read more here.
Keywords: aurora; kinetochore; chromosome segregation; centromere kinetochore ... See more keywords
Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.862637
Abstract: The assembly of a functional kinetochore on centromeric chromatin is necessary to connect chromosomes to the mitotic spindle, ensuring accurate chromosome segregation. This connecting function of the kinetochore presents multiple internal and external structural challenges.… read more here.
Keywords: kinetochore; across evolution; linker; centromeric chromatin ... See more keywords
Centromere and Pericentromere Transcription: Roles and Regulation … in Sickness and in Health
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Genetics"
DOI: 10.3389/fgene.2018.00674
Abstract: The chromosomal loci known as centromeres (CEN) mediate the equal distribution of the duplicated genome between both daughter cells. Specifically, centromeres recruit a protein complex named the kinetochore, that bi-orients the replicated chromosome pairs to… read more here.
Keywords: transcription; centromere pericentromere; kinetochore; transcription roles ... See more keywords