Characterization of essential elements for improved episomal expressions in Kluyveromyces marxianus
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Journal"
DOI: 10.1002/biot.202100382
Abstract: Kluyveromyces marxianus is a promising cell factory for producing heterologous proteins. Essential components including partition element, copy number control element, promoter, and terminator in multicopy plasmids applicable for K. marxianus are not extensively characterized. The… read more here.
Keywords: promoter; kluyveromyces marxianus; pkd1 based; based plasmid ... See more keywords
Hydrolysis of lignocellulose by anaerobic fungi produces free sugars and organic acids for two‐stage fine chemical production with Kluyveromyces marxianus
Sign Up to like & getrecommendations! Published in 2021 at "Biotechnology Progress"
DOI: 10.1002/btpr.3172
Abstract: Development of the bioeconomy is driven by our ability to access the energy‐rich carbon trapped in recalcitrant plant materials. Current strategies to release this carbon rely on expensive enzyme cocktails and physicochemical pretreatment, producing inhibitory… read more here.
Keywords: production; two stage; carbon; kluyveromyces marxianus ... See more keywords
Enhancing bioflavor production by solid‐state fermentation using Kluyveromyces marxianus and l‐phenylalanine
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.202200503
Abstract: This study includes the utilization of sweet lemon peel (SLP) and sugarcane bagasse (SB) in solid‐state fermentation using Kluyveromyces marxianus for bioflavor compounds production adopting response surface methodology. The major flavor compounds, 2‐phenylethanol (2‐PE) and… read more here.
Keywords: fermentation using; using kluyveromyces; kluyveromyces marxianus; production ... See more keywords
Ethanol stress responses of Kluyveromyces marxianus CCT 7735 revealed by proteomic and metabolomic analyses
Sign Up to like & getrecommendations! Published in 2018 at "Antonie van Leeuwenhoek"
DOI: 10.1007/s10482-018-01214-y
Abstract: Kluyveromyces marxianus CCT 7735 offers advantages to ethanol production over Saccharomyces cerevisiae, including thermotolerance and the ability to convert lactose to ethanol. However, its growth is impaired at high ethanol concentrations. Herein we report on… read more here.
Keywords: ethanol stress; marxianus cct; kluyveromyces marxianus; stress responses ... See more keywords
Glycerol production through TPI1 defective Kluyveromyces marxianus at high temperature with glucose, fructose, and xylose as feedstock
Sign Up to like & getrecommendations! Published in 2020 at "Biochemical Engineering Journal"
DOI: 10.1016/j.bej.2020.107689
Abstract: Abstract Glucose, xylose, and fructose from biomass are renewable sources for glycerol production. Here, a TPI1 gene encoding triose phosphate isomerase (TPI) was identified in Kluyveromyces marxianus NBRC1777. The TPI1 gene was deleted in the… read more here.
Keywords: production tpi1; glucose fructose; fructose xylose; glycerol ... See more keywords
Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose.
Sign Up to like & getrecommendations! Published in 2017 at "Bioresource technology"
DOI: 10.1016/j.biortech.2016.11.110
Abstract: Xylose and glucose from lignocellulose are sustainable sources for production of pyruvate, which is the starting material for the synthesis of many drugs and agrochemicals. In this study, the pyruvate decarboxylase gene (KmPDC1) and glycerol-3-phosphate… read more here.
Keywords: kluyveromyces marxianus; xylose glucose; pyruvate production; pyruvate ... See more keywords
Bioethanol production from woody stem Prosopis juliflora using thermo tolerant yeast Kluyveromyces marxianus and its kinetics studies.
Sign Up to like & getrecommendations! Published in 2019 at "Bioresource technology"
DOI: 10.1016/j.biortech.2019.122060
Abstract: In the present study, Kluyveromyces marxianus was utilized to study the batch fermentation kinetics of biomass production, substrate utilization and bioethanol production from woody stem Prosopis juliflora. The pre-treated substrate was subjected to Simultaneous Saccharification… read more here.
Keywords: production; woody stem; kluyveromyces marxianus; production woody ... See more keywords
Rational design of Kluyveromyces marxianus ZJB14056 aldo-keto reductase KmAKR to enhance diastereoselectivity and activity.
Sign Up to like & getrecommendations! Published in 2017 at "Enzyme and microbial technology"
DOI: 10.1016/j.enzmictec.2017.07.012
Abstract: t-Butyl 6-cyano-(3R,5R)-dihydroxyhexanoate ((3R,5R)-1b) is a valuable chiral synthon of atorvastatin calcium. A novel NADPH-specific aldo-keto reductase (AKR) was identified from a thermotolerant yeast Kluyveromyces marxianus ZJB14056 by genome database mining, displaying t-butyl 6-cyano-(5R)-hydroxy-3-oxohexanoate ((5R)-1a) reducing… read more here.
Keywords: aldo keto; diastereoselectivity; marxianus zjb14056; kluyveromyces marxianus ... See more keywords
Soybean residue (okara) fermentation with the yeast Kluyveromyces marxianus
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DOI: 10.1016/j.fbio.2019.100439
Abstract: Abstract The soybean residue (okara) with high moisture content has generally been discarded, which has led to economic loss and socio-environmental problems. The effects of solid state fermentation using Kluyveromyces marxianus on the nutritional components,… read more here.
Keywords: soybean residue; kluyveromyces marxianus; residue okara; fermentation ... See more keywords
Genomic analysis and lactose transporter expression in Kluyveromyces marxianus CCT 7735.
Sign Up to like & getrecommendations! Published in 2019 at "Fungal biology"
DOI: 10.1016/j.funbio.2019.06.004
Abstract: Kluyveromyces marxianus CCT 7735 has been used to produce ethanol, aromatic compounds, enzymes and heterologous proteins besides assimilates lactose as carbon source. Its genome has 10.7 Mb and encodes 4787 genes distributed in 8 nuclear chromosomes… read more here.
Keywords: expression; cct 7735; marxianus cct; lac12 genes ... See more keywords
Co-expression of two heterologous lactate dehydrogenases genes in Kluyveromyces marxianus for l-lactic acid production.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2016.11.015
Abstract: Lactic acid (LA) is a versatile compound used in the food, pharmaceutical, textile, leather, and chemical industries. Biological production of LA is possible by yeast strains expressing a bacterial gene encoding l-lactate dehydrogenase (LDH). Kluyveromyces… read more here.
Keywords: production; kluyveromyces marxianus; expression; ldh enzymes ... See more keywords