MyD88 and not TRIF knockout is sufficient to abolish LPS‐induced inflammatory responses in bone‐derived macrophages
Sign Up to like & getrecommendations! Published in 2023 at "FEBS Letters"
DOI: 10.1002/1873-3468.14616
Abstract: Macrophages play an important role in the response to infection and/or repair of injury in tissues. To examine the NF‐κB pathway in response to an inflammatory stimulus, we used wild‐type bone‐marrow‐derived macrophages (BMDMs) or BMDMs… read more here.
Keywords: trif; derived macrophages; myd88 trif; knockout ... See more keywords
Knockout of tanc2 causes autism‐like behavior and sleep disturbance in zebrafish
Sign Up to like & getrecommendations! Published in 2022 at "Autism Research"
DOI: 10.1002/aur.2880
Abstract: Tanc2 is a large multi‐domain postsynaptic scaffold protein mainly expressed in the brain. In humans, tanc2 mutations have been associated with autism spectrum disorder (ASD) and other related neurodevelopmental disorders. However, the role of tanc2… read more here.
Keywords: knockout tanc2; autism; tanc2 causes; tanc2 knockout ... See more keywords
Effects of inactivation of the cyAbrB2 transcription factor together with glycogen synthesis on cellular metabolism and free fatty acid production in the cyanobacterium Synechocystis sp. PCC 6803
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26842
Abstract: Deletion of the cyAbrB2 (Sll0822) transcription factor in Synechocystis sp. PCC 6803 causes aberrant accumulation of glycogen. We previously tried to redirect the excess carbon stored as glycogen in the cyabrB2‐disrupted (∆ cyabrB2) mutant by… read more here.
Keywords: cyabrb2; pcc 6803; synechocystis pcc; transcription factor ... See more keywords
Online measurement of the viscosity in shake flasks enables monitoring of γ‐PGA production in depolymerase knockout mutants of Bacillus subtilis with the phosphate‐starvation inducible promoter Ppst
Sign Up to like & getrecommendations! Published in 2022 at "Biotechnology Progress"
DOI: 10.1002/btpr.3293
Abstract: Poly‐γ‐glutamic acid (γ‐PGA) is a biopolymer with a wide range of applications, mainly produced using Bacillus strains. The formation and concomitant secretion of γ‐PGA increases the culture broth viscosity, while enzymatic depolymerisation and degradation of… read more here.
Keywords: pga; pga production; shake flasks; knockout ... See more keywords
Producing defucosylated antibodies with enhanced in vitro antibody‐dependent cellular cytotoxicity via FUT8 knockout CHO‐S cells
Sign Up to like & getrecommendations! Published in 2017 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.201600255
Abstract: To engineer a host cell line that produces defucosylated mAbs with superior antibody‐dependent cellular cytotoxicity, we disrupted α‐1, 6 fucosyltransferase (FUT8) gene in CHO‐S (CHO is Chinese hamster ovary) cells by clustered regularly interspaced short… read more here.
Keywords: dependent cellular; knockout; antibody dependent; cellular cytotoxicity ... See more keywords
An efficient and precise method for generating knockout cell lines based on CRISPR‐Cas9 system
Sign Up to like & getrecommendations! Published in 2020 at "Engineering in Life Sciences"
DOI: 10.1002/elsc.202000032
Abstract: Although the efficiency and versatility of CRISPR‐Cas9 system has been greatly improved over conventional genome editing methods such as zinc finger or TALEN, it is still time‐consuming and labor‐intensive for screening knockout/knock‐in cell clones due… read more here.
Keywords: cell lines; crispr cas9; knockout cell; cas9 system ... See more keywords
Endothelial Bmp2 knockout exacerbates hemochromatosis in Hfe knockout mice but not Bmp6 knockout mice.
Sign Up to like & getrecommendations! Published in 2019 at "Hepatology"
DOI: 10.1002/hep.31048
Abstract: Bone morphogenetic proteins BMP2 and BMP6 play key roles in systemic iron homeostasis by regulating production of the iron hormone hepcidin. HFE also regulates hepcidin through a mechanism that intersects with the BMP-SMAD1/5/8 pathway. However,… read more here.
Keywords: hfe; bmp2; mice; bmp6 ... See more keywords
CYR61/CCN1 Regulates Sclerostin Levels and Bone Maintenance
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DOI: 10.1002/jbmr.3394
Abstract: CYR61/CCN1 is a matricellular protein that resides in the extracellular matrix, but serves regulatory rather than structural roles. CYR61/CCN1 is found in mineralized tissues and has been shown to influence bone healing in vivo and… read more here.
Keywords: cyr61 ccn1; bone; expression; cyr61 ... See more keywords
Knockout of NRF2 triggers prostate cancer cells death through ROS modulation and sensitizes to cisplatin
Sign Up to like & getrecommendations! Published in 2022 at "Journal of Cellular Biochemistry"
DOI: 10.1002/jcb.30333
Abstract: Prostate cancer (PCa) represents the second most common cancer in men and affects millions worldwide. Chemotherapy is a common treatment for PCa but the development of resistance is often a problem during therapy. NRF2 (nuclear… read more here.
Keywords: cancer; prostate cancer; knockout nrf2; knockout ... See more keywords
Leucine rich repeat kinase knockout (LRRK KO) mouse model: Linking pathological hallmarks of inherited and sporadic Parkinson's disease
Sign Up to like & getrecommendations! Published in 2018 at "Movement Disorders"
DOI: 10.1002/mds.27267
Abstract: Autosomal dominant mutations in Leucine Rich Repeat Kinase 2 (LRRK2) underlie the most common cause of familial Parkinson’s disease (PD) and have also been associated with sporadic cases. However, LRRK2 mutation knockin and LRRK2 knockout… read more here.
Keywords: dko mice; lrrk dko; lrrk; lrrk2 ... See more keywords
Two ABC transporters are differentially involved in the toxicity of two Bacillus thuringiensis Cry1 toxins to the invasive crop-pest Spodoptera frugiperda (J. E. Smith).
Sign Up to like & getrecommendations! Published in 2020 at "Pest management science"
DOI: 10.1002/ps.6170
Abstract: BACKGROUND The fall armyworm Spodoptera frugiperda is a major agricultural pest that has invaded the East Hemisphere since 2016, generating a serious threat to the food security worldwide including Africa and Asia. The Cry toxins… read more here.
Keywords: abc transporters; spodoptera frugiperda; sfabcc2 sfabcc3; sfabcc2 ... See more keywords