An efficient and precise method for generating knockout cell lines based on CRISPR‐Cas9 system
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DOI: 10.1002/elsc.202000032
Abstract: Although the efficiency and versatility of CRISPR‐Cas9 system has been greatly improved over conventional genome editing methods such as zinc finger or TALEN, it is still time‐consuming and labor‐intensive for screening knockout/knock‐in cell clones due… read more here.
Keywords: cell lines; crispr cas9; knockout cell; cas9 system ... See more keywords
Construction and Transcriptomic Study of Chicken IFNAR1-Knockout Cell Line Reveals the Essential Roles of Cell Growth- and Apoptosis-Related Pathways in Duck Tembusu Virus Infection
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DOI: 10.3390/v14102225
Abstract: For industrial vaccine production, overwhelming the existing antiviral innate immune response dominated by type I interferons (IFN-I) in cells would be a key factor improving the effectiveness and production cost of vaccines. In this study,… read more here.
Keywords: knockout cell; cell; dtmuv; cell line ... See more keywords