Assessment of two CRISPR-Cas9 genome editing protocols for rapid generation of Trypanosoma cruzi gene knockout mutants.
Sign Up to like & getrecommendations! Published in 2018 at "International journal for parasitology"
DOI: 10.1016/j.ijpara.2018.02.002
Abstract: CRISPR/Cas9 technology has been used to edit genomes in a variety of organisms. Using the GP72 gene as a target sequence, we tested two distinct approaches to generate Trypanosoma cruzi knockout mutants using the Cas9… read more here.
Keywords: crispr cas9; gene; trypanosoma cruzi; knockout mutants ... See more keywords
PtAUREO1a and PtAUREO1b knockout mutants of the diatom Phaeodactylum tricornutum are blocked in photoacclimation to blue light.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of plant physiology"
DOI: 10.1016/j.jplph.2017.05.020
Abstract: Aureochromes are blue light receptors specifically found in photosynthetic Stramenopiles (algae). Four different Aureochromes have been identified in the marine diatom Phaeodactylum tricornutum (PtAUREO 1a, 1b, 1c, and 2). Since blue light is necessary for… read more here.
Keywords: blue light; knockout mutants; photoacclimation; phaeodactylum tricornutum ... See more keywords
The Effect of Atmospheric Cold Plasma on Bacterial Stress Responses and Virulence Using Listeria monocytogenes Knockout Mutants
Sign Up to like & getrecommendations! Published in 2019 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2019.02841
Abstract: Listeria monocytogenes is an opportunistic intracellular pathogen commonly associated with serious infections and multiple food-borne outbreaks. In this study, we investigated the influence of atmospheric cold plasma (80 kV, 50 Hz) on L. monocytogenes (EGD-e)… read more here.
Keywords: knockout mutants; treatment; cold plasma; stress ... See more keywords
A Comprehensive Review of Indel Detection Methods for Identification of Zebrafish Knockout Mutants Generated by Genome-Editing Nucleases
Sign Up to like & getrecommendations! Published in 2022 at "Genes"
DOI: 10.3390/genes13050857
Abstract: The use of zebrafish in functional genomics and disease modeling has become popular due to the ease of targeted mutagenesis with genome editing nucleases, i.e., zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and… read more here.
Keywords: genome editing; comprehensive review; indel detection; knockout mutants ... See more keywords