Efficient circular gene knockout system for Burkholderiales strain DSM 7029 and Mycobacterium smegmatis mc2 155.
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DOI: 10.1093/abbs/gmz054
Abstract: Multiple gene knockouts are often employed in studies of microbial physiology and genetics. However, the selective markers that confer antibiotic resistance are generally limited, so it is necessary to remove these resistance genes before the… read more here.
Keywords: gene; knockout system; circular gene; gene knockout ... See more keywords