Histidine is essential for growth of Komagataella phaffii cultured in YPA medium
Sign Up to like & getrecommendations! Published in 2022 at "FEBS Open Bio"
DOI: 10.1002/2211-5463.13408
Abstract: Komagataella phaffii (a.k.a. Pichia pastoris) requires histidine for optimal growth when cultured in a medium containing yeast extract, peptone (YP), and acetate (YPA). We demonstrate that HIS4‐deficient, K. phaffii strain GS115 exhibits a growth defect… read more here.
Keywords: phaffii; komagataella phaffii; growth; cultured ypa ... See more keywords
Metabolic engineering of Komagataella phaffii for synergetic utilization of glucose and glycerol
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DOI: 10.1002/yea.3793
Abstract: Komagataella phaffii GS115 is a proven heterologous expression system and has recently been exploited for the production of value‐added biochemicals from glucose through metabolic engineering. A major challenge for high‐level biochemical production is the appropriate… read more here.
Keywords: phaffii; komagataella phaffii; metabolic engineering; glucose glycerol ... See more keywords
Acetamidase as a dominant recyclable marker for Komagataella phaffii strain engineering
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-8824-9
Abstract: We have investigated the use of the gene coding for acetamidase (amdS) as a recyclable dominant marker for the methylotrophic yeast Komagataella phaffii in order to broaden its genetic toolbox. First, the endogenous constitutive AMD2… read more here.
Keywords: komagataella phaffii; marker; acetamidase; phaffii ... See more keywords
The Mut+ strain of Komagataella phaffii (Pichia pastoris) expresses PAOX1 5 and 10 times faster than Muts and Mut− strains: evidence that formaldehyde or/and formate are true inducers of PAOX1
Sign Up to like & getrecommendations! Published in 2020 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-020-10793-8
Abstract: The methylotrophic yeast Komagataella phaffii is among the most popular hosts for recombinant protein synthesis. Most recombinant proteins have been expressed in the wild-type Mut+ host strain from the methanol-inducible alcohol oxidase (AOX) promoter PAOX1.… read more here.
Keywords: methanol; mut strain; komagataella phaffii; strain ... See more keywords
PiggyBac transposon-mediated mutagenesis and application in yeast Komagataella phaffii
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology Letters"
DOI: 10.1007/s10529-018-2592-6
Abstract: ObjectiveAround one-fourth of the Komagataella phaffii genes encode hypothetical proteins with unknown functions. However, lack of powerful tools for genetic screening in K. phaffii significantly limits the functional analysis of these unknown genes. Transposon mutagenesis… read more here.
Keywords: mutagenesis; piggybac transposon; komagataella phaffii; phaffii ... See more keywords
High-level expression and characterization of Aspergillus niger ATCC 1015 xylanase B in Komagataella phaffii
Sign Up to like & getrecommendations! Published in 2018 at "Applied Biological Chemistry"
DOI: 10.1007/s13765-018-0368-2
Abstract: Owing to the safety issues in food and feed industry, the GH11 xylanase B gene from Aspergillus niger ATCC 1015 was cloned and expressed in Komagataella phaffii. The highest xylanase B activity of 1827.19 U/ml was… read more here.
Keywords: aspergillus niger; high level; xylanase; atcc 1015 ... See more keywords
High-level secretion and characterization of cyclodextrin glycosyltransferase in recombinant Komagataella phaffii.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of biotechnology"
DOI: 10.1016/j.jbiotec.2017.07.031
Abstract: Cyclodextrin glycosyltransferase (CGTase) catalyzes the conversion of starch into cyclodextrin (CD), which is widely applied in food, pharmaceutical, cosmetic, and agricultural industries. For efficient production of CD, high yield of CGTase with good characteristics is… read more here.
Keywords: cyclodextrin; komagataella phaffii; cyclodextrin glycosyltransferase; cgtase ... See more keywords
Engineering a heterologously expressed fructosyltransferase from Aspergillus oryzae N74 in Komagataella phaffii (Pichia pastoris) for kestose production.
Sign Up to like & getrecommendations! Published in 2022 at "New biotechnology"
DOI: 10.1016/j.nbt.2022.02.005
Abstract: Fructo-oligosaccharides (FOS) are one of the most well-studied and commercialized prebiotics. FOS can be obtained either by controlled hydrolysis of inulin or by sucrose transfructosylation. FOS produced from sucrose are typically classified as short-chain FOS… read more here.
Keywords: pichia pastoris; komagataella phaffii; production; oryzae n74 ... See more keywords
Efficient Bioproduction of Human Milk Alpha-Lactalbumin in Komagataella phaffii.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.1c07908
Abstract: Alpha-lactalbumin (α-LA; the most abundant whey protein in human milk) contributes to infant development, providing bioactive peptides and essential amino acids. Here, Komagataella phaffii (K. phaffii) was selected as the production host. We found that… read more here.
Keywords: phaffii; human milk; komagataella phaffii; alpha lactalbumin ... See more keywords
Establishing Komagataella phaffii as a Cell Factory for Efficient Production of Sesquiterpenoid α-Santalene.
Sign Up to like & getrecommendations! Published in 2022 at "Journal of agricultural and food chemistry"
DOI: 10.1021/acs.jafc.2c02353
Abstract: Santalene, a major component of the sandalwood essential oil, is a typical representative of sesquiterpenes and has important applications in medicine, food, flavors, and other fields. Due to the limited supply of natural sandalwood resources,… read more here.
Keywords: cell factory; komagataella phaffii; santalene; production ... See more keywords
Komagataella phaffii YPS1-5 encodes the alpha-factor degrading protease Bar1.
Sign Up to like & getrecommendations! Published in 2020 at "FEMS yeast research"
DOI: 10.1093/femsyr/foaa024
Abstract: Yeast mating pheromones are small secreted peptides required for efficient mating between cells of opposite mating type. Pheromone gradients allow the cells to detect potential mating partners. Secreted pheromone degrading proteases steepen local gradients and… read more here.
Keywords: protease; bar1; komagataella phaffii; pheromone ... See more keywords