Improving the mutagenesis efficiency of the Kunkel method by codon optimization and annealing temperature adjustment.
Sign Up to like & getrecommendations! Published in 2019 at "New biotechnology"
DOI: 10.1016/j.nbt.2019.11.004
Abstract: The Kunkel method is a widely used site-directed mutagenesis strategy that introduces point mutations by annealing mutation-containing oligonucleotides to single-stranded uracil-containing DNA (dU-ssDNA) templates. The method is fast and inexpensive and has been routinely employed… read more here.
Keywords: mutagenesis; point mutations; kunkel method; improving mutagenesis ... See more keywords
Fragment-Directed Random Mutagenesis by the Reverse Kunkel Method.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00086
Abstract: Two fundamentally different approaches are routinely used for protein engineering: user-defined mutagenesis and random mutagenesis, each with its own strengths and weaknesses. Here, we invent a unique mutagenesis protocol, which combines the advantages of user-defined… read more here.
Keywords: kunkel method; mutagenesis; random mutagenesis; method ... See more keywords