Uptake and Fate of Fluorescently Labeled DNA Nanostructures in Cellular Environments: A Cautionary Tale
Sign Up to like & getrecommendations! Published in 2019 at "ACS Central Science"
DOI: 10.1021/acscentsci.9b00174
Abstract: Fluorescent dye labeling of DNA oligonucleotides and nanostructures is one of the most used techniques to track their fate and cellular localization inside cells. Here, we report that intracellular fluorescence, and even FRET signals, cannot… read more here.
Keywords: dna; labeled dna; dna structures; fate ... See more keywords
Preparation of Labeled DNA, RNA, and Oligonucleotide Probes.
Sign Up to like & getrecommendations! Published in 2022 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.top100578
Abstract: Labeled nucleic acids and oligonucleotides are typically generated by enzymatic methods such as end-labeling, random priming, nick translation, in vitro transcription, and variations of the polymerase chain reaction (PCR). Some of these methods place the… read more here.
Keywords: rna oligonucleotide; oligonucleotide probes; labeled dna; preparation labeled ... See more keywords
Fluorescence Quenching of Alexa Fluor 488-labeled DNA by Complementary Trp-containing PNA Partitioned in Liquid-ordered Domains
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DOI: 10.1246/cl.170755
Abstract: We demonstrate Trp-containing PNA/Alexa Fluor 488 (AL488)-labeled DNA hybrid partitions preferentially into liquid-ordered (lo) microdomains of GUV. The Trp quenches the fluorescence of AL488 microscopically. The mechanism involved collisional and static quenching, both of which… read more here.
Keywords: trp containing; labeled dna; trp; fluor 488 ... See more keywords
Detection of fluorescence-labeled DNA with in-plane organic optoelectronic devices.
Sign Up to like & getrecommendations! Published in 2022 at "Biomedical optics express"
DOI: 10.1364/boe.475358
Abstract: We present a system efficiency analysis of a monolithic integrated organic optoelectronic unit for the detection of fluorescence labeled single-stranded DNA (ssDNA) for veterinary disease testing. The side-by-side integration of an organic light emitting diode… read more here.
Keywords: labeled dna; detection; fluorescence labeled; detection fluorescence ... See more keywords