Labeling Antibodies with N-Hydroxysuccinimide-Long Chain (NHS-LC)-Biotin.
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DOI: 10.1101/pdb.prot099259
Abstract: Labeling antibodies with biotin (biotinylation) is a useful and simple technique. Biotin's small size (244 Da) usually has little effect on the biological activity of the protein target. The most common way to biotinylate an… read more here.
Keywords: biotin; labeling antibodies; long chain; antibodies hydroxysuccinimide ... See more keywords
Labeling Antibodies Using a Maleimido Dye.
Sign Up to like & getrecommendations! Published in 2019 at "Cold Spring Harbor protocols"
DOI: 10.1101/pdb.prot099291
Abstract: Fluorophore-maleimide derivatives are effective for labeling sulfhydryl-containing molecules. Maleimide groups react with free thiols at pH 6.5-7.5 forming a covalent bond. Reducing agents should be avoided during the conjugation step. This protocol uses the cross-linker… read more here.
Keywords: antibodies using; using maleimido; labeling antibodies; maleimido dye ... See more keywords